| Broomcorn millet(Panicum miliaceum)is one of the traditional five grains in China.It is drought-resistant and barren-tolerant and is an important crop in arid and semi-arid regions.At present,reference genomes of two broomcorn millet varieties have been published,which are accomplished by whole-genome shotgun sequencing strategy combined with Illumina sequencing technology,PacBio sequencing technology,HiC technology and map technology.The assembly qualities are high.BAC library,as a genomic resource,is of great use in genome sequence improvement,functional gene research and comparative genomics field.The purpose of this research was to integrate BAC library into broomcorn millet genome to maximize the use of BAC library resource.In this study,9216 clones from the broomcorn millet BAC library were chosen as a material set.Using CAPSS(Clone-Array Pooled Shotgun Sequencing)strategy and Illumina sequencing technology,8262 BAC clones were mapped onto the broomcorn millet reference genome,of which 5391 were mapped by paired-end sequences and 2871 by single-end sequence.In order to assess the accuracy,we randomly picked up 55 BAC clones and seuqenced by Sanger method.The loci of 54 BAC clones mapping by Sanger sequences were identical to the former.The average of BAC inserted fragment lengths is 123.48 kb,according to an estimate by the result of BAC paired-end mapping.By aligning the BAC end sequences with the organelle genomes,14 BAC clones from chloroplast genome and no clones from mitochondria genome were identified.Compared to the Sanger sequencing method,our method used in this study can greatly reduce the cost of acquiring BAC end sequences and shorten the period of the experiment.The integration between the BAC library and the genome will facilitate the acquisition of BAC clones with specific functional genes,provide a reliable basis for the correction and improvement of genome sequences,and form the basis of molecular breeding and genomic function research. |
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