Preliminary Function Analysis Of The MtFVE Gene In Medicago Truncatula

Flowering is an important physiological process in the life history of higher plants,and it is an important agronomic trait that affects the successful reproduction and seed reproduction of plants.Plant flowering is affected by different internal and external environmental signals,and is also regulated by complex molecular networks.Studying the flowering time of leguminous plants and plants provides an important reference for the cultivation of legume forages that are more suitable for flowering time.FLOWERING LOCUS VE(FVE),one of the autonomous pathway members in Arabidopsis thaliana,promotes the floral transition by inhibiting transcriptional expression of FLOWERING LOCUS C(FLC)at the chromatin level.FLC acts as a key repressor of floral transition by blocking the expression of integrator genes.Medicago lacks the FLC gene,Therefore,the molecular mechanism underlying the regulation of flowering time by FVE homolog in M.truncatula remains to be elucidated.In this study,the function of MtFVE gene was analyzed by bioinformatics combined with molecular biology method,and the conserved protein interaction in M.truncatula was preliminarily explored by yeast double-hybrid experiment,which further studied the regulation of MtFVE gene.Flowering time provides an important reference value.The main results of the study are as follows:1.Analyzing the homologous proteins of FVE in M.truncatula by Bioinformatics methods,found that two homologs and named as MtFVEa and MtFVEb,respectively;interesting,they both had a typical WD40 protein binding domain.2.To detect the tissue expression of MtFVE gene at transcription level,q RT-PCR analysis revealed that both MtFVEa and MtFVEb were highly expressed in flower and shoot apices in R108 of M.truncatula.Besides,RT-PCR assay also showed that MtFVEa and MtFVEb gene expression patterns were highly consistent with q RT-PCR results,and both genes were highly expressed in flower and shoot apices.3.Subcellular localization analysis of MtFVE,it showed that MtFVEa and MtFVEb were expressed in both nucleus and cytoplasm.4.In order to analyze the functionof MtFVE gene,preliminary phenotypic analysis revealed that the mutants corresponding to MtFVEa and MtFVEb were prematurely flowered compared to the wild type.5.Yeast double-hybrid assay showed that MtFVEa interacted with MtFLD(FLOWERING LOCUS D,a homologous protein of Arabidopsis FLD)and MtHDA6(Histone Deacetylase 6,a homologous protein of Arabidopsis HDA6),suggested that MtFVE might be involved in flowering time regulation by forming the FVE-FLD-HDA6 complex in M.truncatula.The research provided ideas and theory for further research on the function of MtFVE in M.truncatula.