Cloning And Expression Of Cell Wall Protein Genes In Potato

The potato(Solanum tuberosum)has a very important position in the food safety structure.China is one of the major potato producing areas.Potato planting and production have been seriously threatened by Ralstonia solanacearum(bacterial wilt disease)for many years.It is one of the main limiting factors for potato production and stable yield.He pathogen R.solanacearum is a gram-negative plant pathogen that hosts Solanaceae plants,such as potato and tomato,and is a pathogen that causes crop bacterial wilt.2The cell wall structure of a plant is the first cell structure that first perceives external stress signals.Cell wall proteins(CWPs)are present in plant cell wall structures and play an important role in the development of plant cells,morphogenesis,pathogen infection,and environmental response,particularly during growth,development,environmental stresses,and infection,in which plant cell wall-related signal transduction,cell wall relaxation,protease inhibition and other biological functions involved.Plant cell wall protein genes have been shown to involve in plant defense responses.At present,the research on CWP genes was limited to their distribution and structure in potato,and there is little direct evidence as to what theirs functions might be.The study on the gene expression of potato CWPs during the interaction between potato and R.solanacearum would be important for understanding its possible disease resistance.Cell wall proteins exist in the plant cell wall structure and have various biological functions such as resistance to stress,resistance to diseases,signal transduction related to plant cell wall,cell wall relaxation,and protease inhibition.Based on the preliminary work of our laboratory,three CWP genes were cloned from potato(resistance genotype ED13)with the accession numbers EU294357,EU294358,EU294359.Bioinformatics analysis found that the amino acid sequences of the three are highly homologous to the amino acid sequences of other members of Solanaceae.Inoculation experiments combined with bioinformatics analysis showed that R.solanacearum could induce the up-regulation of potato cell wall protein genes.It was concluded that the CWPs were induced by bacteria and involved in carbohydrate metabolism.Intracellular and extracellular signal transduction processes.Three CWPs exhibited different up-regulated expression patterns induced by non-biogenic elicitors such as jasmonic acid,brassinolide,and abscisic acid.q RT-PCR experiments showed that for the responsive reaction,the CWP gene was more More sensitive to brassinosteroids(BR)but mot to the other two plant hormones jasmonic acid(MJ)and abscisic acid(ABA).In situ hybridization analysis revealed that there was no tisue-specific difference in stems and leaves.m RNA signals were found in the vascular bundle system of the above-ground stem tissues and leaf tissues,including phloem and primary xylem.While a certain hybridization signals werealso detected in the epidermis and cortex of stems,these phenomena indicate that the potato CWPs might be expressed in many plant tissues.The mechanism of the up-regulated expression of three potato cell wall proteins induced by biological elicitors and abiotic elicitors,especially the gene interaction network,remains to be further studied.This will greatly expand our understanding of the molecular mechanisms regulating potato growth and development.Our existing results and further research in the next step are expected to provide a theoretical basis for the molecular mechanism of potato bacterial wilt resistance,and have certain significance for potato disease-resistant molecular breeding.