| Zoysia japonica has the characteristics of branPling resistance and rapid recovery growth.In order to explore the mechanism of adaptive growth after mechanical damage,the defense and recovery growth characteristics of Zoysia japonica in response to mechanical damage were studied.By transforming the transcriptome data into ID identified by mapman,we visualized the differentially expressed genes related to eell wall synthesis,beta-1,3-glucanase,Protease,secondary metabolites and transcription factors caused by mechanical damage to Zoysia japonica,and analyzed the changes of hydrogen peroxidase in the antioxidant system to explore the defense mechanism.The response of GA3 and BR to leaf growth regulation in mechanically damaged Zoysia japonica Leaves and the role of GA3 and ABA in promoting leaf primordium growth were studied by fluorescence quantitative method*The following conclusions are drawn:1.The unigenes classified as 20-stress and 26-misc accounted for 1.22%and 1.77%of the total unigenes,respectively.The genes annotated to 26-misc mainly encoded a series of key enzymes,such as glucosyltransferase,beta-l,3-glucan degrading enzyme,antioxidant enzyme,oxygenase and dehydrogenase,and 1871 differentially expressed genes were eo-expressed at 2 h and 6 h to participate in the defense and recovery mechanism of Zoysia japonica.2.Mechanical damage caused rapid expression of a series of recqPtor kinases and different second messenger genes in damaged leaves at 2 and 6 hours.And disease-related proteins and heat shock proteins enhanced resistance to abiotic stresses such as bacterial biological stress and high temperature.3.The damaged leaves rapidly synthesized cell wall precursors and lignin monomers in secondary metabolism at 2h and 6h.There were 155 differentially expressed genes related to cell wall synthesis.Nine differentially expressed genes were up-regulated during the transformation from UDP-glucan to cell wall monomers.34 differentially expressed genes were involved in the transformation from phenylalanine to various lignin monomers.4.149 differentially expressed genes are involved in wax,terpenoids,phenolics and nitrogen compounds produced by secondary metabolic pathways,which are beneficial to defense against the damage of herbivores and oxides.5.There are 26 differe:ntially expressed genes related to beta-1,3-glucanase,which react more intensely at 2 h than at 6 h.There are 138 differentially expressed genes categorized as protease system,while only 55 differentially expressed genes related to ubiquitinated protease system,24 differentially expressed genes related to hydrogen peroxidase are GO-annotated,and CAT and POD are short-term,respectively.Within 2H and 6h,the actively of CAT and POD was higher,so the effect of scavenging H2O2 was obvious,while the reaction activity of APX was not obvious.6.Among the three main transcription factors caused by mechanical damage to leaves,the annotations obtained by loading the transcreptome data into mapman are the same as those of the Swis-Prot database and some Nr database annotations in the transcriptome data.13 differentially expressed genes were found in AP2/EREBP transcription factor,8 differentially expressed genes in NAC transcription factor and 11 differentially expressed genes in WRKY transcription factor.The annotations in the database showed that AP2/EREBP and WRKY transcription factors could enhance the response to abiotic and biotic stresses,enhance stress resistance,and NAC transcription factors could transmit signals to stem meristem and regulate its growth.7.ZjAP2 was involved in the regulation of ABA pathway and GA pathway after 2 hours,and low level of ZjBZR1 stimulated BR synthesis pathway after 2 hours,which made GA and BR participated in the growth process of damaged leaves.8.ABA/GA3 participated in the formation of leaf primordia after 2 hours,and ZjKNOX which coordinated with ZjIPT7 promoted the division of stem cells after 2 hours,which is conducive to the differentiation and formation of leaf primordia. |
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