Analysis Of Genetic Diversity And Differentiation Of Acanthopagrus Schlegelii By AFLP Markers

Acanthopagrus schlegelii is becoming increasingly sought after,not only because of its rich nutrition,rapid growth,large adaptability and high commercial valuable,but also because it is one of the most promising cultured species in China.In recent years,due to the water pollution,overfishing,and other natural environments,the resource capacity and genetic diversity of wild A.schlegelii both sharply declined.In order to protect the wild species,improve the stock quality,keep the development of aquaculture healthy and sustainable,it is necessary for us to study the genetic structure of A.schlegelii.We used AFLP technology to analysis the genetic diversity of ten macrocephlus stocks which captured from Yantai,Weihai,Beihai,Zhanjiang,Zhoushan,Zhangpu,Fuding,Xiamen,Hainan,Nanaodao.A total of 1678 polymorphic loci were amplified from 300 individuals used by 10 primer combinations,the percentage of polymorphic loci was from 63.84%-87.05%,with the average of 74.74%.The mean number of alleles per locus?A?ranged from 1.6384-1.8705,with the average of 1.7470.The mean effective number of alleles?A_e?ranged from1.3090-1.5849.with the average of 1.4347.The mean of Nei's gene diversity?H?was 0.2547,ranging from 0.1946-0.3291.The result shows that genetic diversity?D?level of A.schlegelii high.The results of genetic differentiation analysis show that the mean value of H_s,H_t,G_st,N_m,were 0.2517,0.4187,0.3989 and 0.7535 respectively,and the genetic differentiation level of A.schlegelii is high.The genetic distance among 10 populations ranged from 0.130-0.377,the genetic identify ranged from 0.686-0.878.The UPGMA clustering analysis shows that Yanta,Weihai and Zhoushan belonged one group,and Beihai,Zhanjiang,Zhangpu,Xiamen,Haikou,Nanao and Fuding belonged one group.