Traceability Of Cultivated Lily Maternal Based On Chloroplast Gene Marker And Development Of Lilium DNA Barcode

Lily is not only an important garden flower,an important cut flower and a potted plant,but also widely used for food and medicinal purposes.Among the garden flowers,because of its beautiful flower shape and beautiful flower,it is a plant with great commercial value in garden flowers,and it is also a hot plant repeatedly studied by researchers at home and abroad.From traditional classical taxonomy to modern molecular phylogeny,the origin of Lilium plants has gradually become clear,and the phylogeny has also been contoured.At present,hybrid varieties are continuously cultivated,and the distribution of wild resources is very extensive.Although it is the distribution center of Lilium,the lily breeding started late and is seriously lagging.The hybrid has a large blindness and randomness.Some of the bulbs are imported,which has seriously restricted the development of China's lily industry.How to make use of China's resource advantages and the introduction of lily new germplasm on the basis of introduction is a practical problem that must be solved.The traceability of the cultivated lily varieties is a basic work to be carried out first.Based on the advantages of China's resources,this study is based on the serious lag of lily breeding in China,based on the developed high-resolution chloroplast genome hypervariable region,based on adequate and reasonable sampling to build a reliable phylogenetic relationship,and carry out traceability of the maternal parents of cultivated lily varieties.And the development of Lilium DNA barcodes.The main findings are as follows:1.Based on the chloroplast genome hypervariable region,116 cultivars and 44 wild species were combined to construct phylogenetic trees.The results showed that there were 22 wild species involved in116 cultivars: OT and T series' s original parents are: L.sulphureum,L.henryi;AZ.T/AZ series possible original parents: L.amabile,L.cernuum,L.dauricum,L.leichtlinii var.maximowiczii,L.lancifolium,L.callosum,L.pumilum,L.tsingtauense,L.distichum,L.hansonii,L.longiflorum;possible original parents of O series: L.auratum,L.speciosum var.gloriosoides,L.japnicum;possible original parents of the A series: L.concolor var.pulchellum,L.davidii var.willmottiaee,L.martagon var.pilosiuscu,L.martagon,L.rosthornii.Possible original parents of the LA series: L.brownii,L.formosanum and L.longiflorum and wild species involved in the A series,and also participated in the breeding of LA series cultivars.Wild species not involved in breeding: L.duchartrei,L.lankongense,L.fargesii,L.bakerianum var.aureum,L.lophophorum,L.nepalense var.ochraceum,L.primulinum var.ochraceum,L.taliense,L.bakerianum,L.bakerianum var.aureum,L.bakerianum var.rubrum,L.bakerianum var.delavayi are less involved,although they are mixed with the breed,but there is almost no support rate.(2)Using the chloroplast group hypervariable region to construct the maternal phylogenetic relationship map,and jointly construct a topological structure with high resolution.(3)The hypervariable region sequence was analyzed for the total number of mutations(Eta),haplotype diversity(Hd),nucleotide diversity(Pi),average number of nucleotide differences(k),and number of haplotypes(H)Evaluation of each gene fragment to construct a phylogenetic tree.The psb B-psb H in a single sequence has a high discrimination,and the nuclear gene ITS is the most distinguishable,and the same group of wild species can be brought together.The DNA barcodes combined with ycf1b+mat K+rbc L+ITS and ycf1b+mat K+trn P-psa J-rp I33+ITS are suitable for inter-species identification,and can clearly cluster species with their same species.