Genetic Analysis And QTL Mapping Of Fusarium Head Blight Resistance In Annong 1124

Wheat(Triticum aestivum L.)is a food crop which is widely planted all over the world,and the planting area in our country is extensive and the staple food of the people in northern China..With the global climate and cultivation system changes,Fusarium Head Blight(FHB)has caused more and more serious damage to wheat production in China.So,mining disease resistant genes and breeding have excellent agronomic traits resistant varieties to FHB is the important work for breeding of FHB Resistance in wheat,and it is also the most fundamental way to solve this problem.In this experiment,F2 population and F2.3 family were constructed by hybridization between resistant parent Annong 1124 and susceptible parent Annong 8455,and using single flower drip method population resistance identification,using single flower drip method analysis resistant parent Annong 1124.The F2 population was analyzed by BSA combined with SNP chip and SSR marker and constructed the genetic linkage map to provide a basis for cloning of new genes for FHB resistance and molecular marker-assisted breeding in wheat.The main results of this experiment are as follows:(1)The segregation populations of Annong 1124 and Annong 8455 were constructed,the resistance to FHB of F2 and F2:3 of Annong 1124 was investigated by single flower drip method,the result show:F2(a total of 335 families)population was in accordance with 3:1(χ2=0.67<x20.05,1,df=1,P=0.41>0.05);F2:3 families(a total of 321 families)was consistent with 1:2:1(x2=0.33<x20.05,2,df=2,P=0.85>0.05),it was considered that the resistance to FHB in Annong 1124 was controlled by a single dominant gene.(2)Anti-susceptibility pool was constructed using 660K SNP chip.The results showed that the distribution frequency of different loci in 6A staining was the highest,with 365 SNP loci;two specific CAPS markers P29 and P30 linked to target genes on chromosome 6A were developed.The linkage distances were 41.2 cM and 48.9 cM.(3)Screening of SSR markers on chromosome 6 A,but no markers linked to the target gene were screened.Detection of F2 population using Pm21 gene markers derived from Haynaldia villosa 6VS,the genetic distance between F2 population and target gene was 19.0 cM,linked to the FHB resistance gene in this study,it is presumed that the FHB resistance gene of Annong 1124 originated from Haynaldia villosa parent 92R91,so providing reference information for the comprehensive utilization of resistant variety.