Glucosinolate Accumulation In Brassica Oleracea L. In Response To Feeding Of Spodoptera Exigua And The Effect Of EATP On Glucosinolates In Pakchoi And Arabidopsis Thaliana

Glucosinolates are nitrogen-containing and sulfur-rich secondary metabolites in cruciferous plants and play an important role in plant defense.In this paper,we studied the effects of Spodoptera exigua feeding(biological stress),mechanical damage(abiotic stress)and MeJA on the content of glucosinolates in Brassica oleracea L.,and the combination of glucosinolate synthesis,secondary modification and transport to obtain glucosinolates in Brassica oleracea L..response to S.exigua.ATP in the cells released by the above stress,as an important early danger signal,can induce the enhanced resistance of plants,and it is possible to indirectly regulate the effects of glucosinolates through the downstream JA/ET pathway.The main results are as follows:1.A certain degree of mechanical damage and feeding induction increased Aliphatic glucosinolates content in leaves,but with the increase of feeding harm of Spodoptera exigua,Brassica oleracea L.is more accurate in resisting insect invasion.In addition to the change of the content of glucosinolates related to the synthesis rate of their own glucosinolates,glucosinolates transport may play an important role in it.The change of glucosinolates induced by an exogenous signal MeJA was most similar to that by continuous insect feeding,which indicated that MeJA played an important role in the response of Brassica oleracea L.to beet armyworm invasion.All these provide a basis for further exploring the insect resistance of glucosinolates of Brassica oleracea L..2.eATP,AMP and ATP-Tris treatments significantly changed the contents of Aliphatic and Indolyl glucosinolates in leaves and roots of pakchoi through the synthesis and secondary modification of glucosinolates.To provide research basis for improving the resistance of cruciferous plants to pests and diseases.3.eATP had a significant effect on glucosinolates in wild-type Arabidopsis thaliana leaves.However,there was no significant change in the glucosinolates in the eATP receptor mutant dorn1.Combining the responses of the mutants to eATP,including Aliphatic glucosinolate transcription factor mutants(myb28,myb29),Indolyl glucosinolate transcription factor mutants(myb34,myb51 and myb122),the intracellular ethylene receptor mutant etr1,and med25 which is closely related to the JA pathway,we could conclude that eATP,after being received by DORN1,affects the glucosinolate-related transcription factors and the ET/JA pathways,ultimately leading to changes in glucosinolate content in Arabidopsis thaliana leaves.Studying new signaling molecules through DORN1 may reveal important new details of how plants respond to environmental changes.