Interventional Effect Of Selenium-enriched Yeast On OTA-Induced Intestinal Mucosal Barrier Injury In Broilers

Ochratoxin A(OTA)is a mycotoxin widely present in cereals.The intestine is the first organ to be in contact with OTA and is an important target organ for OTA action.Studies have shown that OTA is particularly harmful to the intestinal tract,and the intestinal toxicity of OTA is mainly oxidative stress and inflammatory response.Selenium(Se)is an essential trace element in the human body.It is the main structural substance of glutathione(GSH),an important antioxidant in the body.It has a very powerful antioxidant function and can remove excess free radicals in the body.Selenium-enriched yeast(SY)is an important seleniumenriching agent,which contains organic selenium which is more easily absorbed by the body.The aim of the study was to determine whether SY interferes with OTA-induced intestinal barrier damage in broilers through the NF-?B signaling pathway.In this experiment,80 broilers of 1 day old age with similar body weight were selected and randomly divided into four groups,20 in each group.Control group,OTA group(50?g/kg.BW OTA),SY group(0.4 mg/kg SY),OTA+SY group(50?g/kg.BW OTA +0.4 mg/kg SY)were continuously administered for 21 days,during which time free Eat water.The body weight of the broiler was recorded during the test,in which the solvent of OTA was sunflower oil and the solvent of SY was physiological saline.After the end of the experiment,the changes of antioxidant index in cecal tissues of broilers were detected.The changes of mRNA and protein expression levels of NF-?B signaling pathway and tight junction-related genes were detected by qPCR and Western Blot.High-throughput sequencing was used to detect cecal bacteria.Diversity.The results showed that:(1)SY played a protective role in the damage of various tissues and organs and the decline of production performance caused by OTA exposure.(2)SY can inhibit the change of oxidative damage ability of cecal tissue in broiler chickens caused by OTA exposure.Compared with OTA group,the content of malondialdehyde(MDA)in cecal tissue is significantly reduced(p<0.01),GSH.The content of the superoxide dismutase(SOD)was significantly increased(p<0.01).(3)SY inhibits the expression of NF-?B mRNA and protein by regulating TLR4/MYD88 signaling pathway,enhances the expression of TJ-related gene Claudin-1 mRNA and protein,and the expression of Occludin and ZO-1 mRNA,and antagonizes OTA exposure.Damage to the intestinal barrier.(4)Through high-throughput sequencing,it was found that OTA caused a decrease in the diversity of cecal intestinal flora in broilers and a decrease in the abundance of beneficial bacteria in the intestine.The above results indicated that SY can alleviate the oxidative damage induced by OTA in cecal broilers,and SY can alleviate OTA-induced intestinal barrier damage in broilers by inhibiting NF-?B signaling pathway.