Screening Of Forage Barley And Preliminary Study On Tillering Mechanism

Barley is the world's tnreeth largest grass crop,with feed,beer,food,etc.Seed is a very high quality animal forage feed.The tillering of barley is a very complicated and cumbersome physiological process involving a combination of various external environmental factors and intrinsic genetic factors.Plant hormones play a very important role in this process.As a new discovered plant hormone in 2008,strigolactone(SLs)has been reported to have a role in regulating plant tillering.At present,there have been some researchs about the regulation of SLs in the tiller of plant,and there have been reports on Arabidopsis,rice,and corn.However,SLs related research in barley is still in its infancy,and the specific biosynthesis and signal transduction pathways are still unknown.Barley is an important crop in wheat crops which germplasm resources are widely sourced and rich in types,screening for forage barley materials It is of great theoretical and practical significance to explore the mechanism of SLs in tiller regulation according to thetiller number.In this study,217 germplasm resources randomly selected from the crop germplasm resource bank of the Chinese Academy of Agricultural Sciences were used as materials to screen out three materials(PZDM03602 ? PZDM00162 ? PZDM08422)with significant differences intiller numbers through two-year and three-point tiller phenotypic statistics.The material's tillering ability,regenerative capacity and biomass were statistically analyzed.It was found that two materials(PZDM00162 and PZDM08422)with good tillering ability has good regenerative ability and higher biomass and luxuriance.It is very suitable as silage.SLs is involved in the regulation of plant tiller.Using the genes of SLs synthesis and signal transduction in plants such as rice and Arabidopsis,and bioinformatics analysis to obtain unicornine lactone synthesis and signaling pathway-related genes in barley,which proved that there are completed SLs synthesis and signaling pathways in barley.Through the analysis of spatiotemporal expression of tissues,it was found that the expression of synthesis of SLs related genes were higher in the roots,which was consistent with the report of SLs synthesis in roots;the expression of SLs signaling regulatory pathway genes in multiple stages and multiple parts of plants.There are both high,indicating that the signal regulation of SLs occurs in the whole plant,and is not limited to the tillering process.Using real-time PCR analysis,it was found that the expression levels of the six genes related to SLs were not directly related to the phenotypes of the three materials obtained,indicating that SLs do not directly regulate tillering in these three materials,and there may be other factors regulate the tillering phenotype of these three materials.This study lays the foundation for the development of barley forage resources,the promotion of rural animal husbandry,the selection of high-quality and efficient green barley materials and the preliminary discussion on the tiller regulation mechanism of barley.