Analysis Of Differentially Expressed Genes In Turmeric Metabolites Based On Transcriptome Sequencing

Turmeric is a perennial herb of the genus curcuma.The roots of turmeric have been used as traditional Chinese medicine for thousands of years.Studies have shown that curcumin has anti-oxidation,anti-tumor,treatment of type 2 diabetes and pharmacological effects on the digestive system.The transcriptome information and genomic information of the genus Curcuma are still not perfect.The quality improvement of the curcuma germplasm in genetic engineering should be monitored by high-throughput methods,and the key genes of regulation should be explored.There are many enzyme genes involved in the biosynthesis of curcumin,but the timing,functional sites and how to play a specific role of gene function are still uncertain.In this study,the quality of curcumin components was screened for different turmeric and eucalyptus germplasms,and the best extraction process was screened.The transcriptome mRNAs of different tissues and different cultivars were screened for differentially expressed genes,and the synthesis of curcuminoids was discovered.Genes,and adding foreign substances for metabolic regulation analysis.The main research results are as follows:1.The optimum process conditions for single extraction of curcumin by ultrasonic assisted extraction:methanol concentration 100%,solid-liquid ratio 1:5(g.mL^-1),extraction time 2 minutes.2.The percentage of curcumin in 12 species of turmeric is 0.03%-1.23%,0.02%-1.22% for demethoxycurcumin and 0.02%-1.5% for dimethoxycurcumin.The content of curcuminoids in GXNN and GY01 germplasm was higher,reaching 6.84% and 3.82%,respectively.3.The clean reads obtained from the transcript sequencing of turmeric obtained 115,587 unigenes by optimizing assembly and splicing.GXNN and FJLY germplasm were screened for 10 differentially expressed genes in flavonoid biosynthesis pathway,45 differentially genes were expressed in phenylpropanoid biosynthesis pathway and 5 differentially genes were expressed in biosynthesis pathways between stilbene,diarylheptane and gingerol.26 differentially expressed genes of flavonoid biosynthesis pathway and 114 differentially expressed genes of phenylpropanoid biosynthesis pathway were screened from leaves and rhizomes.14 differentially genes were expressed in biosynthesis pathways between stilbene,diarylheptane and gingerol.4.The real-time fluorescence quantitative PCR of 15 candidate genes in the turmeric germplasm was consistent with the change trend of the RNA-seq gene expression pattern.The dynamic changes of the genes related to the synthesis of curcuminoids in turmeric and the different germplasm curcumin could be analyzed based on the results of RNA-seq.5.1.00 mmol.L^-1 concentration of exogenous NO donor sodium nitroprusside?SNP?solution promoted the synthesis of curcumin,and 4CL1,HCT1,HCT3,HCT5,PAL1 gene expression decreased,while the expression levels of 4CL2,HCT2,HCT4PAL3,PAL4,CHS2 and CHS4 increased,and the expression of CHS3 did not change.50 mmol.L^-1 NaCl solution inhibited the synthesis of curcumin,and the expression of 4CL1,HCT1,HCT3,HCT5,PAL4,CHS4 gene decreased,PAL1,4CL2,HCT2,HCT4,PAL3,CHS2,CHS3 gene expression increased.