The Effect Of Psoroptes Ovis On The Transcription Level Of Toll-Like Receptors And The Downstream Signal Pathway Molecules In Rabbits

Psoroptes ovis,a chronic and infectious ectoparasitic disease characterized by skin inflammation,scabbing,itching,emaciation and depilation,is parasitic on the skin surface of domestic animals such as sheep,cattle,rabbits and wildlife such as elk,causing huge economic losses to animal husbandry and threatening the survival of wildlife.The triggering of inflammation mainly depends on the recognition of pathogenic microorganisms by Toll-like receptors(TLRs)in the innate immune system,which activates intracellular signal pathways,and ultimately regulates the expression of inflammatory factors,cytokines and co-stimulatory molecules to resist pathogenic infection.At present,there are no studies on TLRs signaling pathways activated by Psoroptes ovis after the infection.Therefore,this study aims to explore the effects of Psoroptes ovis on TLRs signaling pathway related molecules of Oryctolagus cuniculi from in vivo and in vitro,in order to provide basic data for further study of the effects of Psoroptes ovis infection on the innate immune system of the host.1 Expression of TLRs in ear skin and peripheral blood of rabbits with Psoroptes ovisThe transcriptional levels of TLRs(TLR1-6,8-12)in Oryctolagus cuniculi naturally infected with Psoroptes ovis were detected by real-time quantitative PCR.The results showed that compared with healthy rabbits,the expression of TLR4,5 and TLR8,10,11 and 12 in the ear skin of rabbits infected with Psoroptes ovis was significantly increased(P<0.01),while the expression of TLR8,10,11 and 12 was significantly increased(P<0.05),while the expression of TLR1,6 and 9 was not significantly increased(P>0.05),while the expression of TLR3 was significantly decreased(P<0.01)and the expression of TLR2 was decreased,but the expression of TLR8,10,11 and 12 was significantly increased(P < 0.05).Compared with healthy rabbits,the expression of TLR 1,2,4,10,12 and TLR 9 in peripheral blood of rabbits infected with Psoroptes ovis were significantly increased(P<0.01)and significantly increased(P>0.05),but the expression of TLR 6 was down-regulated without statistical difference(P<0.05),but no TLR 3,5,8 and 11 were detected.These results suggest that the transcription levels of TLRs in skin and peripheral blood of the rabbits can be altered by the infection of Psoroptes ovis.2 Expression of TLRs signaling pathway related molecules in the ear skin and peripheral blood of rabbits with Psoroptes ovisThe transcription levels of TLRs signaling pathway related molecules in rabbits naturally infected with Psoroptes ovis were detected by real-time quantitative PCR.Theresults showed that compared with healthy rabbits,the expression of TRIF and IL-8 was significantly increased in the ear skin of rabbits infected with Psoroptes ovis(P <0.01),while the expression of p38,ERK1/2 and NF-?B was increased without statistical difference(P>0.05),while the expression of MyD88 was decreased without statistical difference(P>0.05).Compared with healthy rabbits,the expressions of p38,ERK1/2 and NF-?B in peripheral blood of rabbits infected with Psoroptes ovis increased significantly(P<0.01),IL-8 increased(P<0.05),and TRIF and MyD88 increased(P>0.05).These results suggest that the expression of TLRs signaling pathway-related molecules in rabbit skin and peripheral blood can be altered by Psoroptes ovis infection.3 Effects of excretory-secretory antigen of Psoroptes ovis on TLRs and their signaling pathway-related molecules in primary keratinocytes of rabbitsThe primary keratinocytes of rabbits were used as in vitro models.The effects of excretory-secretory antigen of Psoroptes ovis on TLRs and key molecules of their signaling pathway were detected by real-time quantitative PCR.The results showed that the expression of TLR1,2,3,4,6 and 12 in primary keratinocytes of rabbits stimulated by Psoroptes ovis antigen increased significantly(P<0.01),while the expression of TLR5,8and 9 increased without statistical difference(P>0.05),while the expression of TLR10 and TLR11 was not detected.The expression of MyD88,TRIF,p38,ERK1/2,NF-?B and IL-8in primary keratinocytes of rabbits stimulated by Psoroptes ovis antigen was significantly higher than that of non-stimulated cells(P<0.01).These results suggest that the excretory-secretory antigen of Psoroptes ovis can alter the expression of TLRs and key molecules in primary keratinocytes of rabbits.