The Effect Of Duck-origin Goose Parvovirus On Intestinal Damage And Intestinal Microbiota In Cherry Valley Ducks

In order to investigate the effects of duck-origin goose parvovirus(D-GPV)on intestinal barrier function and intestinal microbiota in Cherry valley ducks,the distribution of D-GPV in intestinal tract,intestinal morphological development,intestinal permeability,expression of tight junction protein and inflammatory cytokines were detected,and Illumina MiSeq sequencing technique was used to analyze the diversity and structure of cecal microbiota and its metabolite short chain fatty acids,for investigating the relationship between the changes of intestinal microbiota and intestinal barrier function in Cherry Valley ducks infected with D-GPV,which has important theoretical and practical significance for further understanding the pathogenic mechanism of D-GPV and the intestinal microbiota of ducks.The results showed that D-GPV mainly infected intestinal epithelial cells and caused the retardation of intestinal morphology and structure and the weakening of epithelial digestion and absorption.D-GPV infection caused the intestinal barrier dysfunction with the increases of intestinal permeability,the decreases expression of ZO-1,Occludin and Claudins-3 and the increase expression of inflammatory cytokines IFN-?,IL-17 a,IL-6,IL-1? and TNF-?.The content of LPS in plasma increased significantly,which induced systemic inflammatory reaction,resulting in a significant increase in the contents of TNF-?and IL-1? in plasma.Diversity of cecal microbiota was reduced and the microbial composition was altered significantly after D-GPV infection,resulting in intestinal microbiota disorder.The ?diversity was significantly lower than that in the control group after D-GPV infection.PCoA,UPGMA cluster analysis and similarity analysis showed that the ? diversity of cecum microbiota in D-GPV infected ducks was significantly different from control group.The results of LEfSe analysis showed that the effect of D-GPV on cecal microbiota was characterized by the decrease of short-chain fatty acid-producing bacteria and the enrichment of potential pathogenic bacteria.In addition,the contents of short chain fatty acids(acetic acid,propionic acid and butyric acid)in cecum content were also significantly decreased.This study showed that D-GPV infection was accompanied by intestinal inflammation and intestinal barrier dysfunction,and the large decrease of beneficial microbiota and the decrease of short-chain fatty acid content in cecum will aggravate the negative effects of D-GPV injury on intestinal tract.Therefore,the change of intestinal microbiota played an important role in the pathogenesis of intestinal damage caused by D-GPV.