| Strawberry was a typical material for studying non-climacteric fruit.In terms of production,?Winter Strawberry? has a higher selling price,so its winter production was closely related to the farmer's planting efficiency.ABA and sucrose were two key factors regulating the maturation of strawberry fruit.At present,the focus of research was also focused on the excavation and mechanism research of ABA and sucrose-regulated key genes in strawberry fruit maturation.In recent years,more and more studies had shown that sucrose and ABA had synergistic effects in regulating the synthesis of anthocyanins and starch.It was still unclear whether ABA and sucrose had a synergistic effect on the regulation of strawberry fruit maturation.In view of this,the study intends to used Bebihoppe‘strawberry as material,firstly to discussed the effects of exogenous sucrose and abscisic acid on the ripening and quality of strawberry fruit in the field,and on this basis,through transcriptome sequencing and analysis of strawberry fruit treated with ABA,sucrose and sucrose + ABA,the key genes of regulating strawberry fruit ripening were further screened out,in order to broaden the understanding of strawberry fruit maturation and regulation mechanisms,as well as the increase of winter yield of strawberries and farmers' income.The main results were as follows: 1.Exogenous sucrose treated strawberry fruits can accelerate the maturation of strawberry fruit in varying degrees,especially treated strawberry at light green,white ripening,and initial-red reriods with100 mM sucrose had the best effect on strawberry fruit ripening,and didn‘t reduce the appearance,intrinsic quality and the content of the main biologically active substances of the full-red strawberry fruit.In addition,light green 150 mM exogenous sucrose treatment significantly increased the fruit weight of mature strawberry fruit;at the same time,exogenous sucrose treated strawberry of the light green period also reduced the titratable acid content of the full-red strawberry fruit,which reduced the acidity of the fruit.Therefore,the 100 mM sucrose-treated strawberry fruit in the light green period was a suitable treatment concentration and period for ensuring the ripening of the strawberry under the premise of guaranteeing strawberry quality.2.Exogenous sucrose,ABA,and sucrose + ABA treatment promoted the ripening of strawberry fruits in light green period,and Exogenous sucrose and sucrose + ABA effectively shortened the ripening period of strawberry fruit about 2 d.In addition,exogenous sucrose,ABA and sucrose + ABA mixed treatment didn‘t reduce the appearance quality,intrinsic quality,main bioactive substance content and antioxidant capacity of the full-red strawberry fruit.Both exogenous sucrose and ABA treatment significantly increased the ascorbic acid and carotenoid content of the full-red strawberry fruits.3.Through the analysis of high-throughput RNA-seq data revealed that ABA and sucrose have synergistic effects on strawberry fruit.Further WGCNA analysis selected two genes,HINT3 and DNAJ,that were significantly associated with ABA treatment,and seven genes that were significantly associated with sucrose + ABA treatment: TC.NCS1,PK,HtpG,GPI,GAPDH,ALDO,and RP-S11 e.The heat map analysis showed that the expression of these seven genes was inhibited in varying degrees on the 8th day after exogenous sucrose,ABA and sucrose + ABA treatment.Among them,the sucrose + ABA treatment had the most obvious inhibitory effect,and 4 of these genes involved in the glycolysis pathway.The experimental results of decreased pyruvate content,down-regulated expression level of FaPK,and Spraying glycolysis inhibitors promoted strawberry fruit coloration further indicated that glycolysis was involved in the process of treated with exogenous sucrose,ABA and sucrose + ABA promoted strawberry fruit ripening. |
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