Epidemiological Investigation Of Multidrug-resistant Shigellosis Isolated From Chikens

In this study,a chicken flocks with diarrhea in JiLin province was isolated and purified,and through the 16 S rDNA sequencing and biochemical methods were used toidentify the isolated bacteria as Shigella,so to identify the isolated bateria as Shigella.The pathogenicity test proved that the isolated bacteria had highly pathogenicity.The drug susceptibility test and MIC test showed that the isolated bacteria were multidrug-resitant bacteria.Phenotypic confirmatory test and drug-resistant gene test showed that the ESBLs of the isolated bacteria were positive,and there were TEM and CTX-M beta-lactam resistance genes in the genome.To exactly diagnose chicken sour hays Shigellosis in some parts of JiLin province,and to provide certain data for effective prevention and treament of the disease.Moreover,there are chicken flocks sampling survey towards 20 fowl-run in different parts of JiLin and collect 200 laying hens' feces,vaccinating in ESBL chromogenic medium differential culture,a total of 82 strains of isolated bacteria.Through the screening of shigella culture medium and the above separation identification method,12 strains of shigella were identified in the isolated bacteria,and the separation rate was 14.6%(12/82).Separation hayes 12 strains of Shigella are able to lead to illness and death of the experimental animals,and have highly pathogenicity.The drug susceptibility results show its are multiple drug-resistant bacteria,the selection 24 kinds of drug susceptibility,can reach the height of the resistance of 16 at most.Only for fosfomycin and fluorine benzene nicol the resistance under 25%,ESBLs are all positive,detection of ?-lactamtype of resistance genes,CTX-M type detection rate was 100%,the TEM detection rate was 92%.