Melon Bacterial Fruit Spot And Cucumber Green Mottle Mosaic Virus Electrochemical Immunodetection Sensor Research

The electrochemical impedance immunosensor utilizes the change in the interface properties of the working electrode to detect the antibody antigen reaction.The electrochemical impedance immunosensing technology is not only simple to use,has a wide detection range,is quick to detect,and does not need to mark tracers,it can automatically output data based on test time,and it also has high sensitivity and high specificity of electrochemical impedance.The change of electron transfer rate at the interface of the modified electrode was detected.With increasing national income,these technologies have broad and bright application prospects in a very wide range of areas such as pharmaceutical engineering,food safety,and environmental quality monitoring.They also have significant research value in the detection of plant pathogens.In this project,an electrochemical workstation was used,which was characterized by excellent interfacial characterization of cyclic voltammetry(CV)and good detection characteristics of the electrochemical impedance spectroscopy(EIS).The antibody was immobilized on the surface of a glassy carbon electrode(GCE)to detect melons of different concentrations.The impedance response of B.catarrhalis and cucumber green mottle mosaic virus is expected to prepare an electrochemical impedance immunosensor that can detect plant pathogens quickly,accurately and sensitively.This article mainly consists of three research components:1.First introduced the significance and research background of the detection of plant pathogens,starting from the construction of principles,methods,objectives,research status and prospects,etc.,systematically summarized the electrochemical immunosensor.Subsequently,electrochemical electrochemical impedance technology in electrochemical immunosensor technology was introduced,including its basic principles,its application status and research progress so far.Finally,the research content and experimental purpose of this topic are briefly described.2.The electrochemical behavior of each modified electrode in the solution of potassium ferricyanide/ferrocyanide potassium was examined by electrochemical voltammetry using a three-electrode system and alternating current impedance method.The appropriate equivalent was selected.The circuit was fitted to the data of the AC impedance spectroscopy and the change in the AC impedance value was used to determine the electrochemical changes that occurred on the surface of the electrode along with the detection.3.The antibody was modified on the surface of a glassy carbon electrode.The electrode surface was placed a series of concentrated of melon bacterial fruit spot and cucumber green mottle virus solution,and the antibody antigen reaction was characterized by cyclic voltammetry.Then the The impedance of charge transfer was was measured by AC impedance method to determine the quantitative detection of target bacteria by the electrochemical impedanceRMs.In this experiment,two kinds of impedance-type immunosensors were prepared to detect melon bacterial fruit spot pathogen and cucumber green mottle mosaic Virus,respectively.There was a good linear relationship between the concentration of melon bacterial leaf spot pathogens and cucumber green mottle virus and charge transfer resistance values.The regression equations were:Y=1262.8X+2266.8 and Y=1129.8X+2011.7.Respectively,R2=0.9729 and R2=0.9718,The minimum detection limit is 0.05 Cfu/ml and can reach 10.240 times cucumber Green mottled mosaic virus solution dilution,respectively,and the detection time was less than 60 min.