Effects Of Xylooligosaccharides On Growth Performance,Nutrient Digestion And Intestinal Microbiota In Weaned Piglets

Experiment 1:180 healthy DLY boars（initial weight is 8.86±0.03kg）were selected and divided into three treatment groups according to a completely randomized block design.Basic diet（control group）,antibiotic diet（basic diet+0.750g/kg chlortetracycline）and XOS diet（basic diet+0.500g/kg XOS）were fed separately.The treatment had 4 replicates（pens）,each with 15 pigs.The whole test was 28d,and each pen was weighed at the initial weight of BW₀,14d BW₁₄,and 28d BW₂₈.Blood collection were on the 14d and 28d.Result showed:XOS significantly improved ADG and ADF of weaning piglets 15-28d（P<0.05）;During1-28d,both XOS and antibiotics significantly increased ADG in weaned piglets,and antibiotics significantly reduced F:G（P<0.05）;In addition,antibiotics significantly reduced blood MDA concentrations,increased T-SOD and GSH-PX concentrations in blood,and XOS significantly increased blood CAT and GSH-PX concentrations（P<0.05）;Besides,on28d,XOS and antibiotics significantly increased blood CAT concentration（P<0.05）.Experiment 2:18 healthy DLY boars（initial body weight:15.02±1.14 kg）were selected and divided into three treatment groups according to a completely randomized block design（refer to test one）and each group consisted of 6 pigs.The trial included a 10-day pre-feeding period and a 4-day stool collection period.Results showed that:XOS had no significant effect on the diarrhea rate of piglets（P>0.10）.Both antibiotics treatments and XOS treatment had a higher calcium absorption in piglets（P<0.05）,and XOS had an increased trend in phosphorus absorption in piglets（P>0.05）.Experiment 3:This experiment detected the morphology of small intestine of piglet from experiment 1 and fecal microorganisms from experiment 2 and we used 16S rRNA high-throughput sequencing technology to analyze the structure of fecal microbial flora.The results showed that antibiotics significantly increased the ileum villi height and villi-crypt ratio（P<0.05）;Antibiotics reduced the fecal microbial diversity to a certain extent,and XOS changed the fecal microbial function to a certain extent;XOS increased the abundance of Lactobacillus,and reduced the harmful bacteria such as Lachnospiraceae,Rikenellaceae,and improve the microbial flora structure to a certain extent.