CAPS Markers And Auxiliary Selection Of Multiple Resistance Materials For Hybrid Offspring Of Cabbage

Using cabbage with high resistance to black rot and Tu MV,early bolting A₂₁ and winter late delay bolting,high susceptibility to black rot and Tu MV P₀₂ as the parent material,cross F₁,F₁generation plants are self-breeding,and the obtained F₃ generation plants and F₄ generation plants are experimental groups for research.Through the differentiation and comparison of the target bands of resistance genes,the late-bolting SCAR markers,black rot resistance SSR markers,and Tu MV SCAR markers of 785 plants of F₃ generation were successfully transformed into unified CAPS markers,and the F₃ generation The field bolting situation of 127 plants was compared with the results of late bolting CAPS markers.The results of F₄ generation of 565 plants infected with black rot in the field and Tu MV infection and the resistance to black rot CAPS markers and resistance to turnip flowers Comparative identification of leaf virus CAPS marker identification results.Finally,the late bolting SC01 marker,black rot resistance C001 marker,Tu MV UC04marker and AS05 marker were obtained.Compared with SCAR markers and SSR markers,CAPS markers are more convenient,fast,accurate,stable,repeatable,and uniform;at the same time,they are unified as CAPS markers and multi-gene polymer plants are selected,which will be better applied to Molecular marker-assisted selection breeding provides sufficient experimental materials for multi-directional molecular marker-assisted selection breeding.The study obtained the following four results:?1?Bolting-resistant SCAR markers,black rot-resistant SSR markers,and Tu MV-resistant SCAR markers of cabbage are transformed into CAPS markers:the superiority of CAPS markers is highlighted in terms of technical means and purpose.Feasibility,necessity,the operation of recovery,cloning,sequencing,primer design,amplification,comparison and other operations of the target band,combined with the natural bolting identification of F₃ generation field plants,and the F₃ generation plant population selected by SCN1/248 primers was delayed bolting The SCAR markers of plants and early bolting plants were transformed into SC01 markers,and the late bolting chain gene CAPS marker was established;by comparing the OL9A03 primer and the USC16/433 primer and ASC13/712 primer,the F₃ generation 785 plant populations were tested for resistance to black rot Plants and plants resistant to Tu MV,accurately convert the OL9A03 marker and USC16/433 marker and ASC13/712 marker to C001 marker,UC04 marker and AS05consistent with the detection results of OL9A03 marker and USC16/433 marke r and ASC13/712marker Marker,the field infection identification results of F₄ generation are basically consistent with the CAPS primer amplification results.Marking methods for late bolting of cabbage,black rot resistance,and resistance to Tu MV resistance were optimized,and molecular marker-assisted selection breeding was further updated and improved,providing a strong basis for future breeding work.?2?Uniform specification of multiple labeling methods:Convert complex and diversified SCAR and SSR markers into a unified,simple,and more advantageous multi-gene aggregation CAPS marker to advance the experimental process and improve experimental efficiency.?3?Multi-gene aggregate plants were selected:1 non-resistant plant was detected in F₃generation plants,31 late bolting and double resistance plants against black rot,172 late bolting and double resistance to Tu MV resistant plants,52 plants resistant to black rot and Tu MV resistance,453 plants with late bolting,black rot resistance and Tu MV resistance.The F₄generation plants obtained from single planting of F₃ generation were subjected to black rot fungus infection and Tu MV infection,and the pre-screened markers of the infection results were compared to identify multi-gene aggregates.The different types of gene aggregate plants screened provide diversified experimental materials for the resistance breeding of cabbage.?4?Molecular marker technology combined with multi-gene aggregates is used in cabbage cabbage breeding work:transforming only a single late bolting plant,black rot resistance or Tu MV-resistant linked genes into three plants in the same plant Resistant linked genes,combined with molecular markers and multi-gene aggregates,are used in cabbage breeding,increasing the efficiency and practicality of hybrid offspring research,shortening the years of cabbage breeding,saving experimental resources,Provide a solid foundation for future research.