| Diamondback moth(Plutella xylostella)is one of the most serious pests in the production of cruciferous vegetables in the world.Meanwhile,its resistance to chemicals and various synthetic pesticides has developed rapidly,causing serious losses to agricultural production.Flubendiamide insecticide is an insecticide that mainly depends on gastric toxicity.In our laboratory,previous research demonstrated that the intestinal symbionts of diamondback moth in sensitive strains and flubendiamide-resistant strains differs in bacterial structure and abundance.Therefore,we speculated that the intestinal symbiont of diamondback moth may mediate the resistance to flubendiamide.Therefore,in this study,we studied the ability of the intestinal flora to degrade flubendiamide by in vitro degradation tests and screened the degrading bacteria,using sensitive strains and flubendiamide-resistant diamondback moth as the research objects.The research results may lay a foundation for further analysis of the interaction between symbiotic bacteria and insect hosts,and provide new ideas for pest control.The main conclusions are as follows:1.Degradation of flubendiamide by intestinal symbionts of diamondback moth on MS medium,and it revealed that Serratia marcescens has the highest ability to degrade flubendiamide among intestinal symbiotic bacteria.2.Four Serratia strains were screened in the degradation test of flubendiamide in MS medium and it showed that the Serratia marcescens(NH6-2)with the highest degradation ability,it could degrade about 52.6% fipronil in 48 h.3.Serratia marcescens(NH6-2)mediated the resistance of diamondback moth to flubendiamide through feeding into the sensitive diamondback moth.4.To test the flubendiamide degradation in soil mixed with Serratia marcescens(NH6-2),it was found that Serratia marcescens(NH6-2)could partially degrade the residual fluorobenzene in the soil,up to 50.26% in 45 days.And it did not affect the growth of plant roots. |
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