| The immune response is a defense reaction that resist the invasion of pathogenic microorganisms in the body.And the invasive pathogens are cleared through a series of physiological processes.Vertebrates have evolved a relatively complete immune system that be divided into the innate immune system and the adaptive immunity system in constant evolution.The innate immune system is the primary defense system for the lower vertebrates.Interleukins are an important class of cytokines and play an important role in inflammatory and immune responses.Interleukins are more widely studied in higher vertebrates,while in fish,they are relatively rare.IL-1?,belonging to the interleukin-1 family,is an important pro-inflammatory factor.And IL-1? is capable of activating downstream signaling molecules for cascade amplification by binding to the corresponding receptors on the cell membrane.MicroRNA(miRNA)that a small molecule single-stranded RNA encoded by the endogenous genes.MiRNAs can bind to target gene mRNA through specific "seed sequences",which can cause mRNA to be degraded or blocked by translation.In recent years,it has been found that miRNAs can participate in the body's innate immune response and act as negative regulators to regulate immune pathway gene expression to prevent over-immunization.Based on the above foundation,we have done following studies:(1)The dual luciferase reporter experiments were demonstrated that miR-144 can act on IL-1?-3'UTR through targeting a "seed sequence".Then,the pre-miR-144 plasmid was constructed,and it was confirmed that the pre-miR-144 had a same action trend as miR-144 mimic,and all of them could negatively regulate the IL-1?-3'UTR.(2)The dual luciferase reporter experiments were demonstrated that miR-144 can target negative regulation of IL-1?,thereby down-regulating IL-1?-activated NF-?B signaling pathway.(3)The western blotting experiment was confirmed that miR-144 inhibits IL-1?mRNA at the post-transcriptional level and affects IL-1? protein expression. |
PDF Full Text Request