The Correlation Between IDD Gene Family And Development Of Shoot/Bud In Phyllostachys Edulis

Moso bamboo(Phyllostachys edulis)belongs to Bambusoideae of Graminaceae,which is the most widely distributed economic bamboo species with the largest planting area in China.The output of bamboo shoots is an important factor related to the benefits of the bamboo industry.Most of the shoots in production are always dormant and do not germinate,which greatly limits their output.According to reports,the IDD transcription factor is highly expressed at the top of shoots and can regulate the development of lateral organs.Therefore,this paper analyzes the Phe IDD gene family from the perspective of bioinformatics and expression patterns and obtains the following results:1.Thirty-two Phe IDD gene family members were identified in the bamboo with complete IDD-domain.Twenty-eight Phe IDD genes have 3 or 4 exons,and four Phe IDD genes have 2 or 6 exons.Gene pairs were found to have similar gene structures.A total of thirty-two Phe IDDs were located on sixteen Scaffolds.2.Phe IDDs showed significant tissue-specific.Seventeen members are highly expressed in roots,three members are highly expressed in stems,ten members are highly expressed in leaves,Phe IDD2 and Phe IDD21 genes are highly expressed in tiller buds.3.81%(26/32)of the Phe IDD genes' s expression patterns were positively correlated with the development process of branches.The expression patterns of twenty Phe IDD genes were positively correlated with the development of underground shoots.The expression pattern of 56.2%(18/32)of Phe IDD genes were positively correlated with the development of both branches and shoots.The expression patterns of the four Phe IDD genes were negatively correlated with the development of both branches and shoots.The Phe IDD1 gene was up-regulated to 39 times during the development of branch buds and shoot buds.Phe IDD2 and Phe IDD21,which are highly expressed at the tillering site,also significantly increased their expression during the development of branch buds and shoots.It is speculated that the three genes Phe IDD1,Phe IDD2 and Phe IDD21 may be related to the development of lateral buds.4.Phe IDD genes showed a rapid response to 6-BA treatment,while IAA and sucrose followed.25%(8/32)of the Phe IDD genes were induced by sucrose,and 9.4%(3/32)of the Phe IDD genes were inhibited by sucrose.15.6%(5/32)of Phe IDD genes were inhibited by 500 ?M IAA,37.5%(12/32)ofPhe IDD genes were induced by 500 ?M IAA.37.5%(12/32)of Phe IDD genes were inhibited by 500?M 6-BA,and 18.8%(6/32)of Phe IDD genes were induced by 500 ?M 6-BA.The Phe IDD21 gene presumed to be involved in the development of lateral buds is induced by sucrose,and responds quickly to hormone treatment.It is indicated that Phe IDD21 gene may be a key factor regulating lateral bud development.In summary,the Phe IDD family genes show significant tissue-specific,and many of them participate in the development of branch buds above ground and germination of shoots.Phe IDD family genes are regulated by sucrose and hormones to varying degrees.Integrating the data,it is speculated that Phe IDD21 gene may be a key factor regulating the development of lateral buds.The results provide a basis for mining the biological function of Phe IDD gene during the development of lateral buds.