| Reversible phosphorylation catalysis by protein phosphatase and protein kinase is one of the main mechanisms of signal transduction in eukaryotes.Sucrose non-fermentation-related protein kinase(Sn RK)is a group of serine/threonine protein kinases that contain a catalytic domain of a typical eukaryotic Ser-Thr kinase and are widely available in plants.The Sn RK gene family is divided into three subgroups in Arabidopsis thaliana: Sn RK1,Sn RK2 and Sn RK3.They are key kinases for many biological processes such as sugar signal transdution,stress response,seedling growth and seed germination in Arabidopsis.Sn RK protein kinase is a central hub that coordinates cellular adaptation to changes in nutritional and energy status and plays a central role in metabolic regulation.Brassica napus is an important oil crop in the world.The seeds of Brassica napus contain a lot of oil and have industrial and edible value.In order to improve the cognition of rapeseed oil metabolism related genes and explore new functional genes,Sn RK gene families in rapeseed were used for the systematic analysis.Finally,92 potential Sn RK genes were used for further analysis.The main results are as follows:(1)In this study,92 BnSnRK family genes were identified in Brassica napus,including 8 Sn RK1 members,23 Sn RK2 members and 61 Sn RK3 members.There were significant differences in isoelectric point,molecular weight and amino acid number of proteins encoded by different subgroup members.GO analysis showed that the molecular function of Sn RK gene family in Brassica napus was mainly reflected in its catalytic activity,and the biological processes involved mainly included stress response,cellular processes and metabolic processes.(2)Analysis of the phylogenetic evolutionary tree of BnSnRK family,gene structure and conserved structural domain,it was found that most members of Sn RK3 subfamily had only 1 or 2 exons and few introns,except members of Sn RK3 subfamily whose sequences were similar to those of Sn RK1 subfamily.A total of 61 conserved motifs were predicted in 92 BnSnRK proteins,8 of which were highly conserved.(3)In the genome of Brassica napus,a total of 119 paralogenetic homologous gene pairs were obtained,and no tandem duplicate genes were found.There are twice as many oil-related genes on chromosome A as on chromosome C.A total of 177 collinear gene pairs were obtained between the genomes of Brassica napus and Brassica oleracea,of which 112 pairs were located on large fragment repeats.A total of 187 collinear gene pairs were obtained between the genomes of Brassica napus and Brassica rapa,of which 115 pairs were located on large segment repeats.Of the 92 BnSnRK members,a total of 77 came from the above large segment repetition,accounting for 83.70% of the family members.(4)Correlation analysis of candidate genes for oil content traits revealed that two genes,Bna A07g12290 D and Bna A10g22850 D,were significantly correlated with oil content.The phenotypic contribution rate of marker s7979 contained in Bna A10g22850 D was the highest(12.95%),and there was a significant difference between the haplotypes of the two genes.Under the condition of p-value=1e-3,we obtained 11 genes which are related to both oil composition and oil content,including Bna A04g15530 D,Bna A06g22800 D,Bna A07g12290 D,Bna A07g20630 D,Bna A07g18050 D,Bna A08g25520 D,Bna A09g51540 D,Bna C02g10730 D,Bna C03g16860 D,Bna C04g06790 D and Bna C04g44390 D.(5)In the analysis of qRT-PCR,the t-test value of relative expression of 5 genes was <0.05,and the relative expression of gene Bna C02g10730 D in the high-oil material was 0.235,with a low expression level.The expression levels of genes Bna A07g12290 D,Bna A10g22850 D,Bna A08g18050 D and Bna C04g44390 D in high-oil materials were 2.854,1.769,4.429 and 116.705 times of those in low-oil materials,respectively,which may positively regulate the oil content of rape seeds. |
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