Systematic Analysis Of BZIP Transcription Factor Gene Family And Preliminary Functional Verification Of PgbZIP48-3 In Jilin Panax Ginseng

As a famous and important medicinal herb in the world,ginseng was known as the "king of herbs".Ginseng contains numerous bioactive components,especially ginsenosides,which are remarkable for mankind's?health.The basic leucine zipper(bZIP)transcription factor is one of the largest and most conservative families of eukaryotic transcription factors.Up to now,bZIP transcription factor has been identified in many plants,and it plays important roles in many biological processes and plant response to abiotic and biotic stresses.Nevertheless,bZIP genes remain unknown in ginseng.In this study,bZIP transcription factors genes in ginseng were identified and analyzed systematically basing on 248,993 Unigenes databases of Jilin ginseng.Furthermore,a gene which closely related to the ginsenoside synthesis was screened and its function was explored.This study fills in the blank of research on bZIP transcription factors in ginseng.The results are as follows:1.We reported 91 bZIP genes identified from ginseng,which was named PgbZIPs.These PgbZIP genes were alternatively spliced into 273 Unigenes.Among the 273 PgbZIP Unigenes,190 Unigenes contained full-length open reading frames(ORF),which were derived from 62 PgbZIP genes.2.Phylogenetic analysis grouped these PgbZIP genes into ten groups,and their evolution was conservative.The motif identification also supported the above results.PgbZIPs protein contained 20 motifs,and PgbZIP in the same groups showed similar motif characteristic.3.Gene Ontology(GO)showed that the PgbZIP genes significantly enriched in four functional categories,including transcription,DNA-templated,regulation of gene expression,nucleic acid binding transcription factor activity and DNA binding.4.The expression analysis showed that PgbZIP Unigenes expressed specifically in 14 tissue parts,42 farm varieties and four different developmental stages,indicating the spatiotemporal specificity of their expression.Interaction analysis showed that PgbZIP Unigenes tended to form a co-expression network,indicating the coordination and interaction between PgbZIP.5.Six PgbZIP genes were selected to perform real time PCR analysis of ginseng under drought stress,which revealed that PgbZIP family genes have an important role in drought stress.6.A gene,PgbZIP48-3,was proved closely related to the content of saponins,and its physical and chemical properties were analyzed.The over expression vector and the interference vector were constructed.7.Transformation of ginseng seedlings were induced by agrobacterium rhizogenes and the ginseng hairy roots were successfully induced.The induction rate of the hairy roots induced by the over expression vector contained PgbZIP48-3 was 1.3% while the induction rate of the hairy roots induced by the interference vector was 1.2%.By detection,the transformation rate of the ginseng hairy roots induced by the over expression vector was 26.3%.8.It was found that overexpression of PgbZIP48-3 gene could affect the content of ginsenoside.