Fine Mapping Of A Major QTL QGpc.ZiSc-2H For Grain Protein Content In Barley(Hordeum Vulgare L.)

Barley?Hordeum vulgare L.?is a important cereal crop and widely used for food,fodder and in beer industry.About 80%of barley products are used for feeding and consumption,and 20%is used for brewing beer.Grain protein concentration?GPC?is the most important factor in determining the utilization of barley.Grain protein content is a typical quantitative trait,QTL mapping and cloning of genes related to grain weight are to elucidate the the genetic and molecular mechanisms of complex yield traits.Which is important means to find the genetic potential of barley yield and improve breeding efficiency.In this study,Based on the initial positioning of the QTL for the grains protein content,the near isogenic lines were constructed with the barley landraces ZGMLEL and Schooner as parents,and the environmental stability QTL site QGpc.ZiSc-2H on chromosome 2H carried out the research of the target interval marker encryption,genetic effect evaluation and fine positioning.The results are as follows:1.In order to verify the genetic effect of grain protein content,the genetic effect of the QGpc.ZiSc-2H locus was performed by using five subisogenic populations derived from heterozygous QGpc.ZiSc-2H segments(BC₃F_3:4)verification.It was proved that the QGpc.ZiSc-2H locus was truly effective in regulating the protein content of the grains,and that the locus of the locus was reduced to a segment with a physical distance of about 23 Mb.2.In order to further narrow down the QGpc.ZiSc-2H localization interval,the flanking markers 2L156 and 2L12 were used to screen the exchange single plants.A total of 39 single plants that had exchanges were identified.Examining the grain protein content of individual plants in 39 F₂ populations derived from the exchange of individual plants,combined with the mark of interval encryption,the target interval was further narrowed to a physical interval of 455Kb between the markers2H-220-2H-910.