Analysis Of Transcriptome And Metabolic Group Of Light-induced Brassica Napus L. In Seedling Stage

Rapeseed is one of the important oil crops.Light has an important effect on the growth and development,yield and quality of rapeseed.Light induction causes anthocyanin accumulation in seedling of rapeseed,and its stem and leaf color becomes darker under strong light.Since anthocyanins have antioxidant and free radical scavenging effects for plants,studies on anthocyanins have received extensive attention.The study of anthocyanin metabolism and molecular mechanism in Arabidopsis is more detailed.However,the study of anthocyanin synthesis mechanism is not clear in Brassica napus L.In this study,the transcriptome and metabolome analysis of light-induced were carried out in order to screen the differentially expressed genes in Brassica napus L.The main findings are as follows:(1)Light induction changed the morphology of rapeseed seedlings,and the color of leaves and stems changed purple under strong light irradiation,and the color of leaves changed obviously.With the extension of induction time,the color of leaves and stems of rapeseed seedlings darker,especially the edge and back color of leaves.It is speculated that the change of leaf and stem color may be related to anthocyanin accumulation.(2)Brassica napus L.(two leaves as one)after weak light and strong light treatment 40.29 Gb Clean Data were obtained.9840 differentially expressed genes were found using B.napus L.as reference genome.Among them,5573 up-regulated genes and 4267 down-regulated genes.Indicating that a series of genes were induced positive expression and negative expression under strong light in seedling of rapeseed.Through GO annotation and KEGG pathway analysis,it was found that differential genes were mainly involved in metabolic pathways,secondary metabolite synthesis and other metabolic pathways.Anthocyanin is a secondary metabolite in plants.The analysis of 51 genes in rapeseed seedlings under strong and weak light is different in anthocyanin biosynthesis pathway.These genes are the key genes to control the color change of rapeseed seedlings in rapeseed anthocyanin biosynthesis pathway.The q RT-PCR verification of 11 genes,such as b HLH28、MYB111、CHS、F3’H、ELIP1、ELIP2、ANS、FLS、ELIP3、ELIP4,proves that the expression of these genes is significantly different under strong light induction.Indicating that these genes are key genes for photoinduced anthocyanin synthesis.(3)Metabolome analysis of rapeseed seedlings(two leaves as one)after weak light and strong light treatment,a total of 864 metabolites were detected and classified,of which 248 were different metabolites,and found that these differential metabolites were classified into 21 categories.The metabolites were analyzed to obtain up to 182 metabolites and down to 66 metabolites.Transcriptome and Metabolome analysis showed that there were multiple enrichment pathways,including 81 metabolites in flavonoid synthesis pathway.There were 7 different metabolites belonging to anthocyanin,which were rosin O-hexoside,anthocyanin,anthocyanin 3-O-glucoside,morning glory pigment 3-glucose,geranium-3-Oglucoside,carvulin,paeoniflorin double glucoside.Taken together,in this study,we analyzed the metabolites and differential genes of light-induced differences,and found 51 differentially genes related to anthocyanin synthesis and 81 differential metabolites.The differential genes were validated.This study is of great significance for the study on anthocyanin synthesis mechanism.