Genetic Diversity Of Tomato Germplasm Revealed By InDel Markers

Tomato(Solanum lycopersicum L.)is a valuable vegetable cultivated worldwide and one of the model plants for biological research.Due to the strict self-pollination and the bottleneck effect of long-term cultivation and domestication,the genetic background of tomato has gradually narrowed.Collection of tomato germplasm resources and systematic research on existing tomato are conducive to improving and enriching the genetic diversity of tomato germplasm.In this study,34 primers with high polymorphic information content(PIC)were selected to analyze the genetic diversity of 139 tomato germplasms,16 agronomic traits were selected to analyze the phenotype of 86 materials with all agronomic traits,and the InDel fingerprint of tomato varieties was constructed.The main results are as follows:1.The principal component analysis and cluster analysis maps of tomato germplasms were obtained based on InDel markers and agronomic traits.According to the genetic relationship,the judgment of advantage combination was provided for the breeders to guide the hybrid breeding.The results successfully separated tomatoes into big-type and cherry-type showed that PCA and UPGMA analysis are useful tools to the study of genetic diversity of tomato germplasm.2.Pearson’s correlation coefficients between pairs of the 16 agronomic traits showed that fruit weight was significantly positively correlated with flesh thickness and the number of ventricles.The correlation between 16 agronomic traits and 34 InDel markers in 86 tomato germplasms revealed that a total of 13 loci were associated with 5 agronomic traits,which could be used in marker-assisted breeding of tomato fruit traits.3.Fingerprint of 19 major tomato cultivars in Shanghai was constructed using 5 InDel primers,which is helpful to identify varieties quickly and accurately,and protect the rights and interests of breeders and farmers effectively.At the same time,a set of computer algorithm for fingerprint construction is constructed to quickly and effectively select the optimal primer combination.These results provide a reference for tomato identification,parental selection and high-quality breeding.