Regulatory Effects Of LncRNA-RNCR3 In Diabetes-induced Retinal Reactive Gliosis

Objective:To investigate the pathogenic role of long noncoding RNA-Retinal Non-coding RNCR3(lncRNA-RNCR3)in diabetes-induced retinal reactive gliosis.Methods:1.Diabetic mouse model was established in vivo by intraperitoneal injection of streptozocin(STZ).The experiment was divided into four groups:normal C57BL/6 mice,STZ-C57BL/6 mice,RNCR3 knockdown STZ-C57BL/6 mice and Scr knockdown STZ-C57BL/6 mice.Immunofluorescence was used to detect the expression of GFAP and vimentin in each group,and visual electrophysiological techniques were used to detect visual impairment.2.High glucose model of Muller cells was established in vitro with high concentration glucose(30mmol/L),and the expression of RNCR3 in Muller cells was down-regulated by shRNA transfection.The experiment was divided into four groups:normal group,high glucose stimulated group,RNCR3 shRNA knockdown with high glucose stimulated group and Scr shRNA knockdown with high glucose stimulated group.MTT,immunofluorescence and qRT-PCR were used to detect cell viability,cell proliferation and the expression of RNCR3,GFAP and vimentin.3.Cytokine antibody array was used to detect relevant cytokines.Regulatory mechanisms of RNCR3 in diabetes-induced Muller cell reactive gliosis was analysed.Results:1.RNCR3 knockdown significantly led to a significant reduction in glial cell reactivity.RNCR3 knockdown could ameliorate visual function.2.RNCR3 knockdown significantly reduced M u Her glial cell viability,decreased the proliferation of Muller glial cells and decreased GFAP and vimentin expression.3.RNCR3 knockdown resulted in a significant reduction in the amount of 10 cytokines,including interleukin IL-2,IL3,IL-4,IL-5,IL-9,IL-13,IL-17,MCP-1,VEGF and TNF-?.Conclusion:RNCR3 knockdown could inhibit retinal glial reactivity,and prevent against high glucose-induced retinal neurodegeneration.This study suggests that RNCR3 is a promising target for the prevention and treatment of diabetes-related neural complication.