| Background;Glucagon is an important hormone released by alpha cells of pancreas which have important role in regulating fat and glucose metabolism.Limited studies are available on glucagon relationship with type 2 diabetes mellitus(T2DM).Therefore this retrospective study was designed.Purpose: To explore the correlation of Glucagon with glycosylated hemoglobin(Hb A1c),body mass index(BMI),waist circumference(WC),fasting c peptide(FCP),total cholesterol(TC),triglyceride(TG),high density lipoprotein(HDL-C),low density lipoprotein(LDL-C),fasting plasma glucose(FPG),2hour glucose(2h PG),age and duration in diabetes patients.Study Design: A retrospective study.Methods: In our retrospective study a total of 402 patients with T2DM(type 2 diabetes mellitus)were collected but among them 120 patients were selected according to the criteria and were divided into 3 groups,40 in each groups with 22 male and 18 female according to their Hb A1 c levels(Group 1 =Hb A1c?7%,Group2 = Hb A1c=7-10%,Group3 =Hb A1 c 310%.They were treated from 1st January 2017 to 30 September 2017 in First Affiliated Hospital of Dalian Medical University in inpatient department of endocrinology.The three groups were similar in age,gender and duration of diabetes.Biochemical parameters including Hb A1 c,BMI,WC,FCP,TC,TG,HDL-C,LDL-C,FPG,2h PG,age and duration of diabetes in diabetes patients were detected.The inclusion criteria were :(1)Age 18-80 years,(2)Hb A1c?7% in group 1,Hb A1 c = 7-10% in group 2,& Hb A1 c ?10% in group 3,(3)Neither previous histories nor characteristics of cardiovascular diseases,cerebral vascular diseases,and peripheral artery diseases,etc.by thorough examination before enrollment.Exclusion criteria for our study were:(1)type 1 diabetes mellitus and other types of diabetes like gestational diabetes etc.(2)cases below 18 years or above 80 years.(3)combined with diabetic acute complications or serious stress within 6 months.(4)Renal failure(serum creatinine>106umol/liter,(5)or hepatic dysfunction(serum ALT>80 U/L).For measure of glucagon,samples of blood were taken in EDTA vial treated with aprotinin(Trasylol 10000KIE/ml;Bayer Healthcare AG 51368)which was added to give a final concentration of 200 KIE per ml of peripheral blood [20].Plasma glucagon values were measured by method of radioimmunoassay which was used in commercially available Kits(Glucagon RIA kit,Millipore,6 Research Park Drive,St.Charles,63304,USA)[20].The intra-assay and interassay coefficients were 1.50 and 3.28,respectively.The results which were continuous variables were presented as mean ± standard error of the mean.The glucagon was normal distribution.The groups were analyzed using analysis of variance(ANOVA).We used Pearson's correlation analysis to identify the factors that had significant relationship with glucagon level.All analyses were per-formed with SPSS software version 21.0(SPSS Inc.,Chicago,IL,USA),and P<0.05 was defined as significant.Results: One Way Anova analysis showed that only fasting plasma glucose and fasting c peptide had significant difference among the 3 groups.It also showed that no significant difference was found for plasma glucagon among the 3 groups.Gender,age and duration of diabetes matched between 3 groups.plasma glucagon level were significantly higher in Hb A1 c group 1(103.82 ±34.29)pg/ml and lowest in group 3(93.39 ± 31.04)pg/ml.Blood glucose control in T2 DM was correlated with beta cell function as FCP and FPG(p<0.05).Correlation showed only glucagon was negatively and significantly correlated with WC(p=0.021,r =-.211*)which means as WC increased glucagon value decreased and vice versa.Conclusions: 1.Blood glucose control in type 2 diabetes patients is correlated with B cell function and fasting plasma glucagon level may not be related with diabetes control.2.Plasma glucagon level of type 2 diabetes patients was significantly negatively correlated with WC which means it might be estimated that the patients with high WC will have less glucagon values and vice versa. |
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