Effects Of Bufei Xiaoji Decoction On Proliferation,Apoptosis And The Expression Of 1L-18 And 1L-1? Of A549 Cells Induced By LPS+ATP

Objective: Bufei Xiaoji Decoction is the basic method for Professor Yuanming Rong to accumulate years of clinical experience in the treatment of lung cancer.It is effective in treating lung cancer.Studies have shown that LPS+ATP can stimulate the growth of lung cancer cells and promote the expression of 1L-18 and IL-1?,while high expression of 1L-18 and 1L-1?,Promote the proliferation of lung cancer cells.In this study,modern research techniques and methods were used to detect the effect of Bufei Xiaoji Decoction on the proliferation and apoptosis of A549 cells stimulated by LPS+ATP and the expression of 1L-18 and 1L-1?.The possible mechanism was also explored.Methods: CCK-8 assay was used to detect the effects of different concentrations of LPS,ATP,CRID3 and Bufei Xiaoji Decoction drug-containing serum on the proliferation of human lung adenocarcinoma A549 cells;Annaxin V-FITC/PI analysis control group,positive control group,different concentrations of supplementation Effects of Bufei Xiaoji Decoction drug-containing serum on the apoptosis of A549 cells;ELISA,q RT-PCR and Western Blot methods were used to detect the expression of IL-1? and IL-18 in A549 cells stimulated by LPS+ATP on Bufei Xiaoji Decoction influences.Statistical analysis was performed using SPSS20.0 software.Graph Pad Prism 6.0 software was used for statistical mapping.Quantitative data were expressed as mean ± Standard deviation(Mean±SEM).Data were analyzed statistically using analysis of variance and paired test.Comparisons among multiple groups were analyzed by one-way analysis of variance(ANOVA).The definition of P<0.05 was considered statistically significant.Results:(1)CCK-8 method: LPS,ATP can promote the proliferation of A549 cells;different concentrations of Bufei Xiaoji Decoction drug-containing serum can inhibit the proliferation of human lung adenocarcinoma A549 cells;positive control drug CRID3 can inhibit human lung adenocarcinoma A549 cells.After proliferation of A549 cells treated with 50 ?M of CRID3 for 24 h,the inhibitory rate was 63.1%;Bufei Xiaoji Decoction inhibited the proliferation of A549 cells stimulated by LPS+ATP,compared with the inhibition of A549 cell proliferation without LPS+ATP stimulation.There was no significant difference between the two groups(P>0.05).Bufei Xiaoji Decoction has obvious inhibitory effect on the proliferation of A549 cells stimulated by LPS+ATP,and has a positive correlation with the concentration and action time of traditional Chinese medicine.(2)Apoptosis of Annaxin V-FITC/PI : Bufei Xiaoji Decoction can promote the apoptosis of A549 cells.Apoptosis and apoptosis rate of A549 cells were observed in 4%,8% and 16% Bufei Xiaoji Decoction drug-containing serumafter 24 h treatment.They were(7.63±3.7)%,(28.07±1.2)%,and(40.25±2.3)%,respectively.The rate of apoptosis was only(1.86±0.75)% in the control group.(3)ELISA and q RT-PCR: Bufei Xiaoji Decoction inhibited the expression of IL-1? and IL-18 in A549 cells stimulated by LPS+ATP,compared with the control group and positive control group(P>0.05).Statistical differences between groups were significant(P<0.05).(4)Western Blot method:Bufei Xiaoji Decoction inhibited the expression of IL-1? and IL-18 in A549 cells stimulated by LPS+ATP.Compared with the control group,the expression of IL-18 protein was significantly different(P<0.05).P<0.05),there was no significant difference in the expression of IL-1? protein(P>0.05).Conclusion(s): Bufei Xiaoji Decoction drug-containing serum can promote the apoptosis of A549 cells and inhibit the proliferation of A549 cells stimulated by LPS+ATP and the expression of IL-1? and IL-18.Bufei Xiaoji Decoction treatment of non-small cell lung cancer The mechanism may be produced by inhibiting the secretion of inflammatory cytokines IL-1? and IL-18 from A549 cells.