Imaging Method Investigation On In Situ Tumor Model Of Ovarian Cancer Of Nude Mice By Micro-CT

Ovarian malignancy is one of the common tumors of the female reproductive system,the incidence rate after cervical cancer and uterine cancer,accounting for gynecological malignancies in the first three.However,due to ovarian cancer deep pelvic,onset concealment,initial symptoms are not obvious,often overlooked,so the vast majority of ovarian cancer diagnosis is already late.The ovarian cancer animal model will be a study of the biological behavior of ovarian cancer and the bowling tool for trial treatment.Common nude mice transplanted tumor model and ascites model and human ovarian cancer because of the natural biological process there is a gap in the practical application of the restrictions.In this study,ovarian cancer cell line SKOV3 was established by in situ transfer technique.In order to non-invasive and continuous observation of ovarian tumors,the mammary gland was imaged using a small animal Micro-CT system.Because the resolution of tissue and organ of Micro-CT system is relatively poor,the imaging parameters are analyzed from the perspective of improving the resolution of tissue and organ.The pharyngeal model of nude mice ovary is designed and simulated for imaging experiment.The By changing the system parameters such as tube voltage and tube current of Micro-CT system,the influence of imaging voltage on the image quality was analyzed quantitatively,and the optimal imaging parameters were obtained.As the gap in the abdominal cavity of the nude mice is small,except the regulation of imaging parameters to improve tissue resolution,contrast-enhanced imaging technology is used.Through the experimental results,we can see that the peritoneal contrast method can effectively fill and enhance the interstitial space in the abdominal cavity,so as to effectively observe the purpose of nude mice ovary.In order to quantitatively analyze morphological changes and volume changes during ovarian tumor growth,the ovaries in abdominal CT images need to be segmented and extracted.In this paper,the ovarian segmentation problem is compared with the problem of liver segmentation,and the existing liver segmentation algorithm is studied and improved.Based on the analysis of the characteristics of ovarian CT and the difficulty of ovarian segmentation,an improved method of ovarian segmentation was proposed based on two classical liver segmentation algorithms.The algorithm has a good segmentation result for the majority of ovary which is slightly adhered with intestinal tracts.The morphological changes of morphology and volume of ovarian tumor were calculated and analyzed.The morphological changes of ovarian tumor were observed and analyzed.With the completion of the above work,the quality of ovarian tumor imaging has improved,but rely entirely on Micro-CT imaging to study the pathogenesis of ovarian cancer is limited by the poor sensitivity,low time resolution.Fluorescence imaging has the advantages of high sensitivity and high temporal resolution.In this paper,the advantages of fluorescence and CT imaging are combined,and some fluorescent materials can be used to generate fluorescence under X-ray excitation.A single source emission X-Fluorescence dual-mode imaging system was used to explore the dual-mode imaging of subcutaneous and in situ tomors in nude mice.From the experimental results,this method can achieve a better three-dimensional dual-mode fusion image of nude mice subcutaneous tumor,ovarian tumor imaging in situ also have some effect.