Effect Of Pkhd1 Gene Deficiency On Fertility In Male Mice

Objective To investigate the effect of PKHD1 gene deficiency on fertility of male mice.Methods 1.10 adult wide-type mice and 10 Pkhd1^-/-mice of same age were taken respectively and epididymal spermatozoa was separated to observe the sperm parameters of the two groups:sperm concentration,vitality,and morphology;2.5 adult wide-type mice and 5 Pkhd1^-/-mice of same age were taken respectively and the wild-type female mice were caged with two groups to observe the fertility indicators:frequency of mating（FM）,rate of pregnancy（RP）and average number of pups（ANP）.Results The deficiency of PKHD1 resulted in low numbers,poor motility,and aberrant morphology compared to wide-type mice（P<0.01）and it could lead to the decline of RP in male mice（P<0.01）.Conclusion Pkhd1 deficiency was sufficient to reduce male fertility in mice.The results of our study provided new insights into a potential complication that is male subfertility in Autosomal Recessive Polycystic Kidney Disease（ARPKD）.