Effects And Mechanisms Of Functional Nanomaterial C60 On Spatial Memory In APP/PS1 Alzheimer’s Disease Model Mice

Objective:To investigate the effects and mechanisms of engineered nanomaterial C60 on spatial memory in APP/PS1 Alzheimer’s disease model miceMethods:Seven-month-old APP/PS1 Alzheimer disease model mice were selected for this study.The experimental group(C60)was given 10 days of continuous intraperitoneal injection of 40ug/ml engineered nanomaterial C60(320ug/kg);Control group(NS)for 10 consecutive days intraperitoneal injection of the same volume of saline.After two days recovering,the experimental group and the control group were then divided into two group,and one group was then conducted for subsequent western blot to detect the Ca MKIIα,Ca MKIIβ and P-Ca MKII levels in the hippocampus(Total),enchylema portion(S),synaptic body(P1)and synaptic membrane(P2).The other group conducted 5 days of continuous Morris Water Maze Training Experiment and the6 th day probe trial.The hippocampal neurons were cultured from the wild-type neonatal rats,and then divided into four groups after seven days incubation;The group A received PBS treatment,group B received C60 treatment,group C received Aβtreatment,and group D received Aβ and C60 treatment.After 3 days of treatment,the changes of Ca MKII protein levels on the synapses were detected by immunofluorescence test.Results: Behavior test results: the escape latencies of the 3rd,4th and 5th days of C60 group was less than that of NS group,but there was no difference between the two groups in the 1th and 2nd days.On the 6th day,the probe trial test results showed that the platform crossings and the duration of the target quadrant of C60 group were greater than that in the NS group,and there was no difference in the swimming speed between the two groups.Western blot results: There was no difference in total part of Ca MKII α,Ca MKIIβ and P-Ca MKII protein levels in C60 group compared to NS Group.The S part of Ca MKIIα and P-Ca MKII protein levels was higher in NS group than C60 group,but the level of Ca MKIIβprotein in NS group was less than C60 group.The P1 part of Ca MKIIαand P-Ca MKII protein levels was higher in NS group than C60 group,but the level of Ca MKIIβprotein in NS group was less than C60 group.The P2 part of Ca MKIIα and P-Ca MKII protein levels was less in NS group than C60 group,but there was no difference on Ca MKII β protein level in these two groups.Immunofluorescence results: There was no difference in the Ca MKII protein levels on synapses in group A compared with that in group B,but the level of Ca MKII protein on synapses in group C was significantly decreased compared with group A and B.The Ca MKII protein level on synapses in group D was significantly improved compared with group C.Conclusion: Engineered nanomaterials C60 can improve the spatial memory of APP/PS1 Alzheimer’s disease mice.Engineered nanomaterials C60 reduced the level of Ca MKIIα and P-Ca MKII protein in the enchylema portion(S)and synaptic body(P1)of hippocampus of APP/PS1 Alzheimer’s disease mice and increased the level of Ca MKIIα and P-Ca MKII protein in the synaptic membrane(P2)of hippocampus of APP/PS1 Alzheimer’s disease mice as well as the level of Ca MKII β protein in the cytoplasm and synaptic body.The engineered nanomaterials of C60 could moderate the adverse effects of Aβ on the reduction of Ca MKII protein level on the synapses in hippocampal neurons of wild type mice.