| Objective: Bladder cancer(BC)is a common urothelial tumor and one of the leading causes of cancer death.BC is divided into muscular invasive bladder cancer(MIBC)and non-muscle invasive bladder cancer(NMIBC).Compared with NMIBC,MIBC is easy to invade and shift.It has been found that Lnc RNA TUG1(Lnc RNA TUG1,taurine upregulation gene 1)plays a key role in the occurrence and progression of various malignancies including BC.However,the specific role of Lnc RNA TUG1 in regulating BC progression is still poorly understood.This study examined the expression of Lnc RNA TUG1 in BC patients’ cancer cells,normal bladder urothelial cells,normal urothelial cells and BC cell lines,and analyzed the differential expression of Lnc RNA TUG1 in BC cells and normal cells.Whether Lnc RNA TUG1 expression is related to BC cell proliferation,migration and invasion,further explore the relationship between Lnc RNA TUG1 and mi R-29 c expression levels in BC cells,and further study the specific mechanism of Lnc RNA TUG1 involved in the occurrence and progression of BC,as bladder cancer(BC)Precise treatment at the target gene molecule level provides new ideas.Methods: Twenty-two bladder cancer tissues from BC patients who had not undergone radiotherapy and chemotherapy and normal bladder tissues from the same patients were randomly selected from the biological sample bank of the First Affiliated Hospital of Anhui Medical University.Then the urinary tract from normal people was obtained from the Chinese Academy of Sciences Cell Bank(Shanghai,China).The skin cell lines(control)and BC cell lines(T24,5637,UMUC-2,and EJ)were transfected with si-TUG1,si-NC,mi R-29 C mimic,or mi R-29 C inhibitors by a cell transfection technique.T24 or EJ cells.Real-time polymerase chain reaction(q RT-PCR)was used to detect the expression of TUG1 and mi RNA-29 in BC tissues and cells.We first altered the expression of TUG1 in T24 and EJ cells by transfecting pc DNA-TUG1 or si-TUG1,and then applied cell viability assays and colony formation assays to verify the relationship between TUG1 and BC cell proliferation.The Transwell method was used to determine cell migration and invasion.Finally,the relationship between TUG1 and mi RNA-29 was determined by double luciferase.Finally,the above experimental results were used to investigate the relationship between TUG1 as an oncogene and BC cell proliferation,migration and invasion.Result:(1)The q RT-PCR method was used to detect the expression of TUG1 in cancer tissue of 22 BC patients.The expression level of TUG1 was higher in BC target cell lines(T24,EJ5,UMUC-2 and 5637).In normal urothelial cell lines.The expression of mi R-29 c was significantly reduced in cancer tissues compared with normal bladder tissue in patients.Compared with normal urothelial cell lines,mi R-29 c expression was significantly reduced in cancer cell lines including EJ,5637,T24,and UMUC-2.(2)In T24 and EJ cells,transfection of pc DNA-TUG1 significantly increased the expression of TUG1,while transfection of si-TUG1 decreased the expression of TUG1;survival and colony formation of T24 and EJ cells transfected with pc DNA-TUG1.Compared with the control group,the viability and colony-forming ability of T24 and EJ cells transfected with si-TUG1 were lower than those of the control group.(3)The migration and invasion abilities of T24 and EJ cells in pc DNA-TUG1 group were significantly increased,and the migration and invasion of T24 and EJ cells in si-TUG1 group were decreased.(4)The mi R-29 c levels of T24 and EJ cells transfected with si-TUG1 were significantly higher than those of T24 and EJ cells transfected with si-NC.The levels of mi R-29 c in T24 and EJ cells transfected with WT-TUG1 were much lower than those in T24 and EJ cells transfected with pc DNA.The Luciferase activity in the mi R-29 c mimic group pmir GLO-WT-TUG1 was significantly lower than that in the mi R-NC group.The luciferase activity in pmir GLO-MUT-TUG1 was unchanged in the mi R-29 c mimic group compared to the mi R-NC group.Conclusion:(1)The high expression of TUG1 and the low expression of mi RNA-29 may be related to the occurrence and progression of BC.(2)Upregulation of TUG1 gene can increase the survival rate and colony formation ability of BC cell line,which indicates that upregulation of TUG1 gene may promote BC cell proliferation.(3)High expression of TUG1 after upregulation can increase the invasion and migration of BC cells.(4)TUG1 and mi RNA-29 interact with each other.High expression of TUG1 inhibits the expression of mi RNA-29 in BC cells.Down-regulation of mi R-29 c reverses the inhibitory effect of si-TUG1 on proliferation,migration and invasion of BC cells. |
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