Effect Of Butterflybush Flower Eye Drops On Expression Of Inflammatory Factors:ICAM-1,IL-6 And IL-17 In The Lacrimal Gland Cells Of Castrated Male Rabbits With Dry Eye Disease

Object:Establishing dry eye model of male rabbits by means of castration to observe the effects of different concentration of butterflybush flower eye drops on the expression of inflammatory factors:ICAM-1,IL-6 and IL-17 in the lacrimal gland cells of castrated male rabbits with dry eye disease,then we can discuss the curative effects.Method:We randomly divided 36 male rabbits into 6 groups,with 6 rabbits in each group:the blank group(A),the model group(B),the low concentration group(C),the middle concentration group(D),the high concentration group(E),the testosterone group(F).In addition to the blank group,all the other male rabbits were excised bilateral testicles and epididymis.From the 9th week after the operation,group C,group D and group E began to use the relative concentration of butterflybush flower eye drops in the eyes,3 times/day.Group F began to be injected Testosterone Propionate Injection 1 time every 3 days.All the male rabbits need to be determined tear film rupture time(BUT)and tear secretion function(Schirmer I,SIT)before the operation,the 8th week after the operation,the 2nd week after the treatment and the 4th week after the treatment.All the experimental rabbits were killed and the lacrimal gland of the both eyes were extracted the 4th week after the treatment.Immunohistochemical method was used to detect the expression of ICAM-1,IL-6 and IL-17.Statistical analysis of obtained data was carried out by SPSS.Results:(1)SIT,BUT:The value of BUT and SIT after the operation was significantly lower than that before the operation,and the difference was statistically significant by self-comparison in group B(P＜0.01);The 4th week after treatment:group B was compared with group C,group D,group E and group F,group E was compared with group C and group D,group F was compared with group C,group D and group E,differences are statistically significant(P<0.05);(2)The omparison of ICAM-1,IL-6 and IL-17 between groups the 4th week after the treatment:the difference of group B was statistically significant compared with the other groups(P ＜0.05);Compared with group C and groupD,the difference of group E was statistically significant(P＜0.05);Compared with group C,group D and group E,the difference of group F was statistically significant(P<0.05);(3)The results of the expression of ICAM-1,IL-6 and IL-17:There was no obvious expression in group A and group F;A large number of brown and yellow granules can be found in the cytoplasm and cell membrane in group B;there was sparse expression in group C,group D and group E.Conclusion:The butterflybush flower eye drops have a similar role to androgens.It can inhibit the expression of inflammatory factor ICAM-1,IL-6 and IL-17,so it has the effect of treating dry eyes,but it is less effective than androgens.Among the different concentrations of butterflybush flower eye drops,high concentration of butterflybush flower eye drops has the best effect on dry eyes.