Experiment Research Of Three-dimensional Printed Hydroxyapatite/Polylactic Acid Composite On The Repairment Of Rabbit Cranial Defect

Objective: Exploring and observing the feasibility of applying to the rabbits cranial defect on the Three-dimensional Printed of HA/PLA composite.Methods: Using 3D printing to copy the composite implants of HA/PLA.selecting 12 New Zealand rabbits as experimental animals.Then,preparing 2 Full-thickness defects which is about 1cm of its diameters on the cranial of each rabbit.We divided 2 defects into defect A and defect B.In the defect A,we put the autologous bone powder which was collected during the operation as the control group.In the defect B,we put the composite implants of HA/PLA which were already printed out as the experimental group.After operation,the 6 New Zealand rabbits which were died in 6 weeks,and the others were died in 12 weeks.We picked out and analyzed the samples,observed those samples in morphology?the test of hardness as well as histopathology.Results: 1.the skull defects were repaired and filled well through both the 3D printing of HA/PLA network composites and the autologous bone powder in 6 weeks and 12 weeks.2.In the 6th week,the microhardness in defect B is lower than the part of defect A.(P<0.05).In the 12 th week,the difference between the two repairing parts which were compared with the normal bone microhardness is meaningless in statistics(P>0.05).3.From the experimental group,the new bone narrow cavity and bone cells were formed in the pore surface of the HA/PLA scaffold.The trabeculae formed well and the trabeculae were arranged along the defect direction.Conclusion: 3D printing HA/PLA network composites implants has the characteristics of better biocompatibility,induced bone regeneration and biodegradability.This material could repair the skull defects effectively.