Study On 3d Reconstruction And Migration Of CD133-positive Neural Stem Cells In Postnatal Mice Brain

Neurogenesis in the mammalian central nervous system is believed to end in the period just after birth.With the deepening of the research,we found the adult mammalian brain harbors new neurons throughout life in two discrete locations:subventricular zone(SVZ)of the lateral ventricle and subgranular zone(SGZ)of the dentate gyrus.NSCs will migrate to the sites of injury where nervous system occurred damage,ischemia or aberrant cell cycle regulation.CD133,also called Prominin-1,is one of key biomarkers for NSCs in the central nervous system.CD133+NSCs isolated from the postnatal and adult mammalian brain that can grow as neurospheres.In the previous work,we revealed the existence of dormant ependymal NSCs throughout the ventricular surface of the CNS,as well as signals abundant after injury for their activation.Therefore,the research of CD133+NSCs has important implications for the development of mammalian nervous system and disease treatment.In this article,we analyze the neurogenesis in mammals brain and the distribution of CD133~+NSCs.On the one hand,we use of genetically modified tracer mice as animal model and in combination with tissue optical clearing and light sheet fluorescence microscopy obtain the slicing images of mouse brain.Then we structured the three-dimensional(3D)reconstruction model of the postnatal mouse brain use of 3D reconstruction technology,and to observe the distribution and migration of CD133~+cells in the whole mouse brain.We found that the CD133~+cells is widespread in mouse brain,and the main distribution area is lateral ventricle,three and four ventricles.On the other hand,we used of immunohistochemical staining the specific location of mouse brain.we found that CD133~+NSCs locate in subependymal zone of lateral ventricle,three and four ventricles,and we observed the migration of CD133~+cells between three and four ventricles.The results of this study may provide a feasible experiment for nervous system development,neurodegenerative diseases,and brain injury research.