The Mechanism And Effect Of MiR-99a On The Cellular Proliferation In Cervical Cancer HeLa Cells

[Objective]:MiRNAs are closely related to cervical cancer.It is helpful to understand the molecular mechanism of cervical cancer.This study aimed to investigate the mechanisms on miR-99 a affect the proliferation of cervical cancer HeLa cells and to provide experimental basis for elucidating the molecular mechanisms of cervical cancer.[Methods]: Firstly,miR-99 a mimics was introduced into the HeLa cells by transient transfection,and the proliferation of HeLa cells was observed by cell growth curve and forming assay.Secondly,the HOXA1 gene 3 'UTR targeting regulated by miR-99 a was predicted by Targetscan 6.2 software.Thirdly,after miR-99 a mimics was introduced into the HeLa cells,the expression of HOXA1 gene was analyzed by the luciferase reporter gene,q RT-PCR and Western-blot.Lastly,the interference vector of HOXA1 was constructed and transfected into the HeLa cells.The proliferation of HeLa cells affected by the HOXA1 expression was observed by cell growth curve and cloning experiment.[Results]: 1.After miR-99 a mimics were introduced into the HeLa cells,miR-99 a slowed down the growth and decreased the proliferation ability in HeLa cells the clones number of miR-99 a mimics VS miR-control:156±25 VS 386±40(F=26.093,P=0.001).2.miR-99 a combined with the 1485-1497 nucleotide site of HOXA1 gene 3 'UTR.Moreover,the expression of HOXA1 gene was inhibited by miR-99 a in HeLa cells.3.After the expression of HOXA1 gene was interfered,the growth of HeLa cells was slowed down and the proliferation was decreased the clones number of si-HOXA1 VS si-control: 160±25 VS 390±32(P<0.05).[Conclusion]: MiR-99 a combined with the 1485-1497 nucleotide sites of HOXA1 gene 3 'UTR targeting silenced its expression to inhibit the proliferation of cervical cancer HeLa cells.