Study On The Expression Of LncRNAs And Their Correlation With Clinical Manifestations In PBMC Of SLE Patients

Objective:Long non-coding RNA(IncRNA)is a type of functional RNA molecule located in the nucleus and/or cytoplasm that is more than 200 nucleotides in length and lacks the function of encoding proteins.It can regulate gene's wide participation in the body's physiology and pathological process at epigenetic level,transcription level and post-transcriptional level.IncRNA shows a significant regulatory role in cell differentiation and in the body's immune response,with the development of various sequencing technologies,IncRNAs have been significantly promoted in human diseases.Defining disease-associated IncRNAs in SLE patients is helpful for establishing new biomarkers and potential therapeutic targets.It also lays a certain theoretical foundation for further studying the specific mechanism between IncRNAs and SLEs and searching for disease susceptibility genes.This issue will explore the differences in the expression of IncRNA in peripheral blood mononuclear cells(PBMC)of patients with SLE and its relationship with the disease.Method:This study divided into two groups,SLE group(SLE)and healthy control group(NC).13 cases of SLE patients and healthy participants were collected peripheral venous blood 20ml,after total RNA was extracted from PBMC of two groups of subjects and combined with linc00861 differentially expressed in SLE patients screened by high-throughput sequencing(RNA-seq)and HISAT,String Tieand Blotting analysis,qRT-PCR further validate linc00861 and analyze its association with the clinical manifestations of SLE disease.Result:1.The high-throughput sequencing technology(RNA-seq)analysis of 63677 Ensemb1 gene ID gene expression matrix,then final 11044 gene symbol expression matrix,there are 1584 IncRNAs.The expression matrix was transformed into logarithm,the final lncRMAs that were significantly differentially expressed in SLE patients were predominantly linc00861.2.The RT-qPCR results showed that the expression of linc00861 in SLE patients was significantly lower than that in healthy controls(NC group),the difference was statistically significant(P<0.05).3.The correlation between the expression level of linc00861 and its clinical manifestations in patients with SLE was analyzed.It was found that the expression level of linc00861 in PBMC of SLE patients was negatively correlated with the SLEDAI score;the expression level of linc00861 in patients with proteinuria was significantly lower than that in patients with proteinuria;linc00861 The expression level is lower than that of patients with normal complement of SLE.Cnclusion:The expression level of linc00861 in PBMCs of SLE patients was significantly lower than that of normal controls and correlated with disease activity and degree of renal involvement,suggesting that linc00861 may be involved in the pathogenesis of SLE.The study of the pathogenesis of SLE suggests a new direction and helps to establish new organisms.Mark and provide potential therapeutic targets.