Preliminary Exploration Of Correlation Between Head And Neck Squamous Cell Carcinoma And Human Papilloma Virus

Background:Currently,HPV infections in head and neck squamous cell carcinoma patients reported from around the world have different infection rates and infection types in different regions.The infection status of different countries,ethnic groups and regions is very different and there have not been a report on the situation of HPV Infection in Jilin Province.Many studies have pointed out that the occurrence and development of some head and neck carcinomas have relationship with HPV infection,especially with high-risk HPV infection.Some authors also found that these HPV-related head and neck squamous cell carcinomas are significantly different from non-HPV-related head and neck squamous cell carcinoma in survival,prognosis,and response to treatment.Objective:In this experiment,tissue samples from patients with head and neck squamous cell carcinoma in Jilin Province were selected to detect HPV infection rate and HPV virus subtypes in patients with head and neck squamous cell carcinoma in Jilin Province.In addition,by analyzing the clinical data of HPV-positive group and HPV-negative group to compare their differences in the response to radiotherapyMethods and Materials:1.The tissue specimens was collected from patients diagnosed with head and neck squamous cell carcinoma at the Second Hospital of Jilin University from December 2015 to December 20172.Real-time PCR amplification was performed on tissue samples using HPV E6/E7 specific primers to detect 85 cases of head and neck squamous cell carcinoma(There were 47 cases of laryngeal cancer,14 cases of oropharyngeal cancer,3 cases of oral cancer,and 21 cases of hypopharyngeal cancer.)and 40 paracancerous tissues to detect the infections of HPV16,HPV18,HPV33,HPV51,and HPV52.3.Patients were grouped according to HPV infection status.According to the general clinical data of the two groups of patients and radiological data before and after radiotherapy,the patients were classified as TRG1,TRG2,and TRG3 according to the TRG criteria proposed by Becker.The TRG1 can be divided into For TRG1 a and TRG1b,then compare the grading results of the two groups of patients to assess the sensitivity of the two groups to response of radiotherapy.Results:11 cases(12.9%)of HPV positive patients were detected in 85 cases of head and neck squamous cell carcinoma,in which 10 cases were detected of HPV16,and the other one was HPV33;Of the 47 patients with laryngeal cancer,5 were found to be positive(10.6%)and all were HPV16;Four of 14 patients with oropharyngeal cancer were positive(28.6%),3 were HPV16,and 1 was HPV33;Two of the 21 patients with hypopharyngeal cancer were positive(9.5%)and all were HPV16;Positive results were not detected in 3 patients with oral cancer and 40 paracancerous specimens.A total of 5 patients in the HPV-positive group were treated with radiotherapy,all of which reached TRG1,of which 3 reached TRG1 a and 2 reached TRG1 b.In the HPV-negative group,19 patients received radiotherapy,and 8(42.1%)of them reached TRG1.4 reached TRG1 a,4 reached TRG1 b,9(47.4%)reached TRG2,and 2 reached TRG3(10.5%)Conclusion:1.The HPV positive rate of head and neck squamous cell carcinoma in Jilin was 12.9%,which was lower than that reported at domestic and abroad.HPV16 was the main virus subtype.2.HPV-positive patients with head and neck squamous cell carcinoma have significantly better response to radiotherapy than HPV-negative patients(p=0.028)...