Synthesis Of Novel 2’-deoxy-ginsenoside Rh₂ For Glycolysis Inhibitor And Screening Of Its Anti-tumor Activity

Ginseng is the dry root and rhizome of the ginseng of Araliaceae.It is one of the most precious Chinese medicinal materials in China.It has the functions of improving immunity,improving cardiovascular,regulating the central nervous system,lowering blood sugar,delaying senility,resisting cancer,whitening,and resisting oxidation.Many aspects play a significant role.The ginsenoside triterpene saponins,which are the main active ingredients of ginseng,play an important role in inhibiting tumor growth,promoting tumor cell apoptosis,inhibiting tumor angiogenesis,etc.,and can overcome common adverse reactions of chemotherapeutic drugs and are very promising.Anticancer drug candidate molecules.Therefore,the use of Dammarane-type ginsenoside as a lead compound for structural modification provides an important basis for the development of new antitumor drugs.On the basis of the previous work of this research group,this dissertation uses the total ginsenosides as raw materials and uses the method of degradation to separate and purify 20（S）-protopanaxadiol.The new ginsenoside derivative utilizes the existing 20（S）-protopanaxadiol as a raw material,2-deoxy-D-glucose protected,selective deacetylation,addition,coupling,deprotection and other steps After the reaction,the target compound,2’-deoxy ginsenoside Rh₂,was obtained.In this experimental method,the raw materials are economically available,and the product yield is high and the side reactions are small.Determination of target compound against human cervical cancer HeLa,human gastric cancer cell SGC7901,human hepatoma cell HepG-2,human prostate cancer cell PC-3,human pancreatic adenocarcinoma cell Bx Pc-3,human colon cancer cell HCT-using thiazolyl blue（MTT）method 116,Growth inhibition of colon cancer cell line SW620,human non-small cell lung cancer cell A549,human breast cancer cell MCF-7 and human malignant melanoma cells.Preliminary results showed that the new glycolytic inhibitor 2’-deoxy ginsenoside Rh₂ on human cervical cancer HeLa,human gastric cancer cell SGC7901,human hepatoma cell HepG2,human prostate cancer cell PC-3,human pancreatic adenocarcinoma cell BxPc-3,Human colon cancer cells HCT-116,human colon cancer cell SW620,human non-small cell lung cancer cell A549,human breast cancer cell MCF-7 and human malignant melanoma cell proliferation have different degrees of inhibition,in which the target compound Cervical cancer cells,non-small cell lung cancer and breast cancer have strong inhibitory effects.