Study On The Correlation Between APOE Gene Polymorphism And Hypertensive Cerebral Hemorrhage

Objectives Through the analysis of hypertension cerebral hemorrhage and ApoE gene polymorphism research,from the gene level to discuss the relationship between disease and gene polymorphism,for clinical identification of HICH high-risk patients and provide evidence for treatment strategies.Methods HICH group:55 patients with primary hypertensive intracerebral hemorrhage in affiliated hospital of north China university of science and technology were selected,and all the selected patients were in accordance with the guidelines for diagnosis and treatment of intracerebral hemorrhage in China in 1995.Exclusion criteria:parenchymal hemorrhage of hypertension patients with arteriovenous malformation,hemorrhage caused by venous sinus thrombosis,bleeding after subarachnoid hemorrhage,cerebral infarction,trauma,drugs,blood disease caused by cerebral hemorrhage,and without the head CT diagnosis,all does not belong to the research object,should be excluded;Patients with severe primary diseases such as liver and kidney dysfunction and hematopoietic system should be excluded.Patients with alzheimer’s disease were also excluded from the use of liposuction before admission.HBP group:20 cases of hypertension patients hospitalized in our hospital during the same period,excluding abnormal liver and kidney function,vascular dementia and alzheimer’s disease,history of diabetes,history of hyperlipidemia,etc.Pregnant and lactating women;Alcohol,drug abuse,psychotropic substance abuse.And to ensure that none of the above subjects were related.Healthy control group,45 patients with selected session at the same time the hospital physical examination,eliminate kidney dysfunction,vascular dementia and alzheimer’s disease,and diabetes history,history of high cholesterol,high blood pressure,etc.Pregnant and lactating women;Alcohol,drug abuse,psychotropic substance abuse.And to ensure that none of the above subjects were related.All the subjects signed the informed consent form and passed the examination and approval of the ethics committee of the affiliated hospital of north China university of technology.All of the above research object were extracted DNA,and through real-time fluorescent quantitative PCR and DNA sequencing to detect all the ApoE gene type,and then compare group,through the analysis of the different distribution of ApoE gene type,discusses the influence of ApoE gene polymorphisms on hypertensive cerebral hemorrhage.Results 1 In the HICH group,ε2/ε4 genotype distribution was the most(32.7%),followed byε3/ε3 andε3/ε4.In HBP group,ε3/ε3 genotype was the most common(30.0%),followed byε2/ε3,ε3/ε4,and noε2/ε2 homozygous genotype.ε3/ε3 was the most common in healthy control group(62.2%),and noε2/ε2 homozygous genotype was found.Statistical analysis was performed in the three groups,χ~2=20.349,P=0.026(P<0.05),so the genotype frequency difference between the three groups was statistically significant.ε2/ε4 genotype frequency was 15.0%in HBP group.The frequency ofε2/ε4 genotypes in HICH group was the highest,accounting for 32.7%.Theε2/ε4 genotype frequency in the healthy control group was 8.9%.ε2/ε4 genotypes in HICH group were significantly higher than those in the control group.2 Theε3 allele frequency was the most common in HICH group,43.6%,followed byε4 allele(29.1%),and finallyε2 allele(27.3%).The most common allele in the healthy control group wasε3(75.6%),followed byε4 alleles(13.3%)andε2 alleles(11.1%).Theε3 allele frequency in HBP group was the highest(55.5%),followed byε4allele(25%),andε2 allele frequency(20%).The chi-square test was performed on the frequency distribution of the three groups of genes,andχ~2=10.442,P=0.034(P<0.05),with significant difference,and statistically significant,andε2 andε4 allele frequencies in HICH group were significantly higher than those in the control group.There was no significant difference in genotype frequency between male and female patients(P>0.05),suggesting that the distribution of ApoE genotype in the population may be independent of gender.HICH onset age range from low to high:ε4/ε4<ε2/ε4<ε3/ε4<ε2/ε2<ε2/ε3<ε3/ε3.The three genotypes containing the epsilon 4 gene onset age there are significant differences between groups,epsilon 4 carriers onset age was significantly less than the epsilon 4 carriers,with the increase of epsilon 4 gene quantity,gradually reduce the onset age.3 The statistical analysis of HDL showed that there was no significant difference between the three groups(P>0.05).Compared with the control group,TC,TG and LDL were significantly increased(P<0.05).4 Through the comparison of three different allele carriers of blood lipids,only different allele carriers of TC,TG values have significant difference(P<0.05),compared withε3 allelic genome,ε2 andε4 allelic genome TC,TG levels increased significantly,there were no significant differences between the rest.Conclusions 1ε2/ε4 genotype is a high-risk group of HICH,among which,the most common ones are theε2 andε4 alleles,suggesting that theε2 andε4 alleles may be the susceptibility factors of HICH.2 The genotype containing theε4 allele of the gene maybe can reduce the age of onset of HICH,and decrease with the increase of the number of patients with the increase in the number of patients.3 ApoE was involved in blood lipid metabolism,where TC and TG values were significantly increased in theε2 andε4 alleles,which provided the conditions for HICH to occur.