Expression Of RIP140 In Human Hepatocellular Carcinoma And Its Clinical Significance

Objective: To investigate the expression of receptor interacting protein 140(RIP140)in human hepatocellular carcinoma(HCC)tissues and to analysis its clinical significance.Methods: Immunohistochemistry detected the expression of RIP140 in fifty-four HCC tissues and para-cancerous tissues;Analyzed the relationship between the expression RIP140 in HCC tissues and clinicopathological features of HCC;Western blot and q RT-PCR techniques were used to detect that protein and m RNA expression of RIP140 in nineteen pairs of frozen HCC tissues and para-cancerous tissues;To analyze the relationship between RIP140 expression in HCC and postoperative survival.Results: Immunohistochemical shows that RIP140 was mainly located in nucleus and the expression of RIP140 in HCC tissues was significantly lower than that in para-cancerous tissues;The positive rate of RIP140 expression was 29.7±5.3% in HCC and 92.3±6.1% in para-cancerous tissues(P<0.05);The expression level of RIP140 in HCC tissues was closely related to the serum alpha fetoprotein(AFP)level and whether the tumor invasion blood vessels or not.there was no statistical correlation between RIP140 and TNM staging of HCC and other clinicopathological features;The expression of RIP140 protein and m RNA in HCC tissues was significantly lower than that in para-cancerous tissues(P<0.05);The expression RIP140 in HCC tissues was negatively correlated with postoperative survival.Conclusion: RIP140 was low expression in HCC,and its low expression was significantly related to serum AFP,tumor invasion blood vessels and postoperative survival.RIP140 could be used as an independent prognostic factor for HCC patients,and provide a reference for the evaluation of postoperative prognosis of HCC patients.