Effect Of Picroside ? On The Mitochondrial Permeability After Cerebral Ischemia/Reperfusion In Rats

Objective:The aimis to study the effects picroside II(PicII)on the expression of voltagedependent anion channel 1(VDAC1)and the mitochondrial permeability,and explore the neuroprotective effect of Pic II after ischemia/reperfusion in rats.Methods: Totally 140 successfully Wistar rat models of middle cerebral artery occlusion/reperfusion(MCAO/R)were divided into the model group,the picroside II(Pic II)group,the ruthenium red(Ru R)group,Ru R+ Pic II group,the Spermine(Sper)group,Sper+Pic II group(n=20).After ischemia 2h/reperfusion 24 h,the neurobehavioral function was observed by modified neurological severity scale(m NSS).The cerebral infarction volume was detected by triphenyltetrazolium chloride(TTC)staining.The contents of reactive oxygen species(ROS)and(BDNF)in brain tissues were measured by enzyme-linked immunosorbent assay(ELISA).The permeability of m PTP was assayed with spectrophotomet.The ultra-structure of neurons was examined by transmission electron microscopy(TEM).The morphology and apoptotic cells of brain tissues were observed by hematoxylin-eosin staining(HE)and terminal deoxynucleotidyl transferase d UTP nick end labeling assay(TUNEL),respectively.The expressions of Endo G and voltage-dependent anion channel 1(VDAC1)were determined by immunohistochemistry(IHC)and Western blot.Results: Compared with the shame group,the m NSS scores,the opening degree of m PTP,the contents of ROS,the apoptosis of neuron,and the expressions of VDAC1 and Endo G in the cytosol and nuclear were enhanced in the model group(P<0.05).But mitochondrial Endo G and BDNF content were decreased,and the mitochondria structure was seriously destroyed(P<0.05).The Pic II group exhibited slight decrease in m NSS scores,and ROS content,number of apoptotic cells,m PTP permeability,expressions of VDAC1 and Endo G in cytoplasm and nuclei,the morphology and ultra-structure of brain tissue was improved compared with the model group(P< 0.05).Conclusion: Picroside II could attenuate cerebral ischemia/reperfusion injury by downregulating the expression of VDAC1 and decrease the permeability of m PTP to inhibit the Endo G release from mitochondria into cytoplasm.