Effect Of Curcumin Combined With Sorafenib To Proliferation And Autophagy In Vitro Hepatocellular Carcinoma HepG-2 Cells

Objective: This experiment mainly explored the inhibitory effect of single drug Curcumin,single drug Sorafenib and the combination of two drugs on the proliferation of hepatocellular carcinoma cells and whether it could induce autophagy in hepatocarcinoma cells through the PI3 K / AKT / m TOR signaling pathway and whether the combination of the two drugs A synergistic effect.Methods: Hep G-2 cells with logarithmic growth phase and no contamination were selected as research cells.Hep G-2 cells were treated by different concentrations of Curcumin(0,10,20,30,40,50mmol/L),Sorafenib(0,5,10,15,20?mol/L)alone for 24 h.CCK-8 assay was used to detect the inhibition of proliferation of Hep G-2 cells.Then Hep G-2 cells were treated by single drug Curcumin 30mmol/L,single drug Sorafenib 10?mol/L and the combination of two drugs for 24 h.CCK-8 assay was used to detect the inhibition of proliferation of Hep G-2 cells.Hep G-2 cells were treated by single drug Curcumin 30mmol/L,single drug Sorafenib 10?mol/L and the combination of two drugs for 24 h.And Real-time PCR was used to detect m RNA expression of key proteins AKT,m TOR in PI3K/AKT/m TOR signaling pathway and autophagy-related protein LC3-II.Results:CCK-8 assay was used to detect the effects of blank control group,single-agent Curcumin,single-agent Sorafenib and combination therapy on the proliferation of Hep G-2 cells.The results showed that single-agent Curcumin,single-agent Sorafenib,and combination therapy all had inhibitory effects on the proliferation of Hep G-2 cells and the inhibitory effects on the proliferation of Hep G-2 cells was increased with the increase of the concentration.Compared with using the blank control group,Curcumin or Sorafenib alone,the combination group had more abilities to depressing the cell proliferation and the difference was statistically significant(P<0.001).At the same time,the results of real-time PCR indicated that the PI3K/AKT/m TOR of autophagy-related signaling pathway in Hep G-2 cells treated with single-agent Curcumin,single-agent Sorafenib and two-drug combination.The m RNA expression levels of key proteins AKT and m TOR were decreased compared with the blank control group,while the m RNA expression of autophagy-related protein LC3-II was increased compared with the blank control group,and the change was most significant in the combination group(P<0.001).Conclusion: Curcumin combined with Sorafenib can effectively depress the cell proliferation in Vitro Hepatocellular Carcinoma Hep G-2 Cells and maybe induce cell autophagy through PI3K/AKT/ m TOR signal pathway.