The Expression And Clinical Significance Of TRAIL And Its Receptor In The Granulosa Cells Of Patients With Polycystic Ovary Syndrome

Background: Polycystic ovary syndrome(PCOS)is one of the most common gynecological endocrine diseases,the incidence rate of women of childbearing age and adolescent is about 5%-10%.The pathogenesis of PCOS is very complicated,many studies have found that PCOS is a diseases caused by the interaction between multiple genes and environmental factors.In recent years,more and more scholars have found that apoptosis is also an important factor in the pathogenesis of PCOS.A variety of apoptosis regulating factors,such as Bcl-2,Bax,Fas,Fas L,p53 and Caspase1,3,9 and so on,are involved in the regulation process of granulosa cell apoptosis,thereby affecting the pathogenesis of PCOS.Some scholars have found that tumor necrosis factor related apoptosis inducing ligand(TNF-related apoptosis inducing ligand,TRAIL)and its receptor--death receptor 4(death receptor 4,DR4(decoy),decoy receptor 1 receptor 1,Dc R1)are abnormally expressed in granulosa cells of PCOS rats.At present,there are few studies about TRAIL and its receptor DR4 and Dc R1 in polycystic ovary syndrome.Therefore,detecting the expression of TRAIL and its receptor DR4 and Dc R1 in granulosa cells of PCOS is very important for exploring the pathogenesis of PCOS.Objective:1.The aim of this study is to detect the expression of TRAIL and its receptor DR4 and Dc R1 in granulosa cells of PCOS patients,and to clarify the expression characteristics of TRAIL and its receptor DR4 and Dc R1 in PCOS patients.2.To analyze the difference in the expression of TRAIL and its receptor DR4 and Dc R1 between PCOS and control in groups and explore its relationship with pathogenesis of PCOS.Methods: This study included 30 patients with PCOS and 30 control subjects who are receiving IVF-ET in the Reproductive Department of the Affiliated Hospital of Qingdao University from August 2016 to April 2017.Collect the relevant clinical data.Ovarian granulosa cells and follicle fluid were collected from two groups on the day of oocyte retrieval.RT-PCR was employed to analyze the expression of TRAIL and its receptors DR4 and Dc R1 in two groups,Western-blot was employed to examine the expression of TRAIL protein、DR4 protein and Dc R1 protein.Result:1.Clinical parameters :There were no statistically significant difference in the age,E2,FSH,P,BMI,TSH,days of GN,endometrial thickness on the trigger day between two group(P > 0.05)).based LH,T,E2 and the follicle number above 1.4cm on trigger day,number of oocyte and high quality embryos in the PCOS group were higher than those in control group,the difference was statistically significant(P < 0.05)).RTPCR analysis showed that the expression of TRAIL m RNA in PCOS ovarian granulosa cells was significantly higher than that in control group(P>0.01),the expression of DR4 m RNA of the two groups had no significant difference(P>0.05),the expression of Dc R1 m RNA was significantly reduced than the control group(P<0.01).2.Western blot results indicated that the expression of TRAIL protein in PCOS group was higher than that in the control group(P<0.05),and the DR4 protein PCOS group was higher than that in the control group(P>0.05),and the Dc R1 protein significantly decreased in the PCOS group(P<0.05).Conclusion: 1.TRAIL was highly expressed in granulosa cells of PCOS patients and Dc R1 was lowly expressed in granulosa cells of PCOS patients.2.Abnormal expression of TRAIL and Dc R1 may contribute to the pathogenesis of PCOS and affect the outcome of pregnancy by regulating the apoptosis of granulosa cells.