Role Of Common Inflammatory Factors And AECA In BALF In The Pathogenesis Of Autoimmune Emphysemaof Rats And SAM Intervention

Objective:To study the role of tumor necrosis factor-?(TNF-?),interleukin-8(IL-8),matrix metalloproteinase-9(MMP-9)and anti-vascular endothelial cell antibody(AECA)in the pathogenesis of autoimmune emphysema.To observe the effects of S-adenosylmethionine(SAM)on the inflammatory factors and AECA in autoimmune emphysematous rats.Methods: Thirty SD rats were randomly divided into three groups: normal control group(n = 10),autoimmune emphysema group(n = 10)and SAM intervention group(n = 10).The autoimmune emphysema group was treated with intraperitoneal injection of human umbilical vein endothelial cells plus complete Freund's adjuvant.SAM intervention group was treated with intraperitoneal injection of human umbilical vein endothelial cells,complete Freund's adjuvant and SAM,while the normal control group only with equal volume of liquid(Without any drug)and complete Freund's adjuvant.Twenty-one days later,the lung tissue sections were stained with hematoxylin and eosin(HE)to observe the morphological changes.Mean liner interval(MLI)and mean alveolar number(MAN)were quantitatively measured.The left lung was subjected to alveolar lavage to prepare BALF.The levels of TNF-?,IL-8,MMP-9 and AECA in BALF were detected by ELISA.Results:(1)The MLI of model group was higher than that of normal control group(P<0.05),while MAN was decreased(P <0.05).Compared with model group,MLI in intervention group decreased(P <0.05)and MAN increased(P <0.05).The MLI of intervention group was higher than that of normal control group(P <0.05),while MAN was decreased(P <0.05).(2)The levels of TNF-?,MMP-9 and AECA in BALF in model group were significantly higher than those in normal control group(P <0.05).(3)The levels of TNF-? and AECA in BALF in intervention group were lower than those in model group(P<0.05),but AECA in BALF of intervention group was higher than that of normal control group(P<0.05).(4)There was no significant difference in the concentration of IL-8 among the three groups(P> 0.05).Conclusions:(1)Elevated TNF-?,MMP-9 and AECA concentrations may be involved in the formation of autoimmune emphysema of rats.However,IL-8 may not be related to the disease.(2)SAM may inhibit the formation of autoimmune emphysema of rats by decreasing the production of TNF-? and AECA.