Correlation Analysis Of SLC2A9 Gene And SLC22A12 Gene With Primary IgA Nephropathy Complicated With Hyperuricemia

Objective: To investigate the correlation between primary IgA nephropathy and hyperuricemia and the correlation between SLC2A9 gene and SLC22A12 gene and uric acid excretion.Methods: A total of 153 Han Chinese patients with primary IgA nephropathy diagnosed by renal biopsy from January 2017 to January 2018 in the Department of Nephrology,First Affiliated Hospital of China Medical University were selected.Among them,hyperuricemia was included in the trial group(72 persons)and the normal blood uric acid group were included in the normal group(81 persons);The general population status,lifestyle disease history,family history,and previous medication status were recorded in the form of a questionnaire.And we collect the physical measurement index and laboratory biochemical index information;whole blood genomic DNA was extracted,and the target gene fragment was amplified by PCR.The amplified product was subjected to single-nucleotide polymorphism detection by high-temperature ligase detection reaction(LDR).Finally,statistical analysis was made on IgA nephropathy with hyperuricemia and the association between single nucleotide polymorphisms of rs3733591,rs11722228 of SLC2A9 gene and rs7932775 and rs893006 of SLC22A12 gene and IgA nephropathy complicating hyperuricemia.Result:(1)The sex,24-hour urinary protein quantification,serum creatinine,urea nitrogen,cystatin-C,serum uric acid,uric acid clearance,creatinine clearance,and e GFR were statistically significant between the experimental group and the control group(P <0.05).Katafuchi score in mesangial proliferation,spheroids sclerosis,interstitial cell infiltration,interstitial fibrosis,tubular atrophy,vascular wall thickening,glomerular score,renal tubular score,interstitial score,vascular score,total score There was an equal difference between groups,and the experimental group scored higher than the control group.(P<0.05)(2)Increased urea nitrogen,cystatin C,creatinine clearance,and severe balloon adhesion can lead to high levels of blood uric acid,while men,younger age,non-smoking history,low levels of uric acid clearance,and uric acid excretion scores,e GFR can lead to elevated levels of uric acid.(3)Patients with hyperuricemia had a reduction in the fraction of uric acid excretion.The rs3733591 locus allele was different between the experimental group and the control group(chi-square = 7.39,P = 0.025),and there was no statistical difference in the rest sites(P>0.05).(4)The CC/CT+TT genetic model of rs7932775 locus may have statistically significant differences between the experimental group and the control group(P=0.068),OR=2.5(0.914-6.839),TT/CC+CT model of rs3733591 locus exists differences between groups(P<0.05),OR=2.465(1.246-4.875),and there was no statistical difference in other genetic models.(5)The CC/CT+TT genetic model of rs7932775 in male patients was different between groups.The genetic model of rs3733591TT/CC+CT was different between groups(P<0.05).There was no difference between rs11722228 and rs893006 between groups.There was no statistical difference between female patients(P>0.05)(6)The rs7932775 locus was associated with the BMI of the general patient and the normal uric acid group,and was associated with the degree of mesangial cell proliferation in the hyperuricemia group;the rs3733591 locus was associated with the uric acid and hyaline degeneration of the overall patient,and the BMI of the normal uric acid group.And associated with TC and LDL-D in hyperuricemia;rs11722228 was associated with mesangial proliferation in hyperuricemia group;rs893006 was associated with BMI in normal uric acid group,also correlated IgA leve and serum uric acid in hyperuricemia group(P<0.05).(7)There was no significant difference between the genotype groups in the fractionation group of uric acid excretion.The rs3733591TT/CT+CC expression pattern differed between groups.There was no significant difference between the genotype groups in the fractionation group of clearance of uric acid.The rs893006 TT/GT+GG expression pattern differed between groups.(8)The carrying G allele of rs893006,the C gene of 11722228,the T gene of rs7932775 and the C gene of rs3733591 are high-risk individuals with IgA nephropathy complicating hyperuricemia.Rs3733591 and rs11722228 have a strong synergy,synergy of rs7932775 and rs893006 is weak,and the synergy with the other two sites is also weak.In the experimental group,the high risk gene composition ratio of the control group wasdifferent(P=0),and the incidence of hyperuricemia in the high gene carrier was 7.840 times that of the low risk genome.Conclusions:(1)The onset and development of IgA nephropathy are closely related to hyperuricemia.(2)The rs3733591 locus was associated with the onset of hyperuricemia in IgA nephropathy,rs7932775 locus may be associated with the onset of hyperuricemia in IgA nephropathy,and the rs3733591 locus and rs7932775 locus were associated with the onset of hyperuricemia in males.(3)In the hyperuricemia group,the fraction of uric acid excretion was mainly reduced,and the rs3733591 locus affected the fraction of uric acid excretion in patients,resulting in a decrease in uric acid excretion.The rs893006 affects the rate of uric acid excretion in patients,but this study did not clearly reveal the specific role.(4)The risk of hyperuricemia with high-risk genotypes was 7.840 times that of low-risk genotypes.The synergism between rs3733591 and rs11722228 of the SLC2A9 gene was stronger;the synergy between the SLC22A12 genes rs7932775,rs893006 was weaker,and SLC2A9 The synergy of the other two sites of the gene is also weaker.