Extraction And Separation Of Chemical Constituents Of Curculigo,and Screening Of Its Antitumor Activity

Objective:Curculigo as a Chinese medicine commonly used in traditional Chinese medicine,has a variety of effects,but over the years at home and abroad on its cell-level pharmacological research is not a lot,this topic aims to use traditional methods and traditional methods of column chromatography,recrystallization separation The main monomer compounds of Curculigoside were identified and analyzed,and the characteristic compounds with higher contents were screened for activity of the tumor cells and the study of antioxidation,so as to explore the purpose of preliminary pharmacological activity,In order to provide the theoretical and data basis for the clinical application of monomer compounds of Curculigo or Curculigoside in the future.Methods:The 15 kg Curculigo repens extract was heated repeatedly with 70% ethanol for several times and then concentrated by cyclohexane,ethyl acetate and n-butanol respectively.The extract was concentrated to an extract and purified by silica gel column,ODS Column,polyamide column,gel column,recrystallization and so on were used to separate and purify the ethyl acetate fraction and the n-butanol fraction,respectively,and the chemical structure of the purified monomer compounds was confirmed by means of Poplar analysis.The four high-priority monomeric compounds were isolated and their inhibitory effects on the proliferation of breast cancer MCF-7 cells and Hep-2 cells were detected by CCK-8 assay.By flow cytometry,tumor cells were induced to induce apoptosis in the compound in which the tumor suppressing effect was good.Results:Thirteen compounds were extracted from C.cinnabarinus,of which six compounds were identified by spectral analysis,namely curculin,curculoside B,curculigoside C,?-sitosterol,?-glucoside and rutin.The remaining compounds have not identified their structure.The primary pharmacological activity test was carried out by the CCK-8 test method.Curculigoside,curculoside B,and glucosyl syringate had different degrees of growth inhibitory effects on human breast cancer cell MCF-7 cells,whereas curculoside B and curculigo sp.The glycoside C also had different degrees of growth inhibitory effect on human hepatoma cells Hep G2 cells in a time-and dose-dependent manner(P<0.05).Flow cytometry confirmed that curculoside B has a certain degree of ability to induce apoptosis of tumor cells.Through preliminary pharmacological tests,curculicidin,curculoside B,and glucose syringate have different degrees of growth inhibitory effects on human breast cancer cell MCF-7 cells,while curculoside B and curculigoside C are also on human hepatoma cells.Hep2 cells had different degrees of growth inhibitory effect in a timeand dose-dependent manner(P<0.05).It has been experimentally confirmed that curculoside B has a certain degree of ability to induce apoptosis of tumor cells.Conclusions: Through different experimental methods,four higher-content compounds were isolated and identified from Curculigo crassifolia,namely,curculoside,curculoside B,curculigoside C,and glucose syringate.Among them,Curcumin B,Curculigoside C and Glucose syringate all have potential antitumor activity and induce apoptosis of tumor cells,which provides scientific basis and research direction for anti-tumor research of Curculigo.