| Objective:(1)To detect changing of kidney function and kidney diseased that shiweijiangtang pulvis(SP)treat diabetic nephropathy SD rat,exploring the protective effects of SP on kidney of DN rats.(2)CTGF was detected when sp treating process of rat tenal fibrosis caused by diabetes,and to provide theoretical basis for sp treatment rats with DN.Methods: Thirty healthy male SD rats were selected to our experiment.after one week,Ten rats were randomly selected as the normal control group with common feed and not any special processing.Then The remaining 20 rats were established diabetic nephropathy model,First,the rats were fed for four weeks with high sugar and high fat forage.Then injected stz,the Dose is 45mg/kg according to the weght of rats,establishment of rat model of early DN standard :(1)Fasting blood glucose level above 16.7 mmol/L.(2)Urine protein in rats was positive after 2 weeks.DN rats were fed high sugar and high fat forage,we randomly selected 10 rats as model control group and anther 10 as SP group.according to rat weight,the model control group served saline solution(0.003ml/g)and the SPgroup served shiweijiangtang pulvis(0.003ml/g).Each group rats urine is collected to detected urinary micro-albumin values in weeks 2nd,6,10.Tail vein blood test fasting blood glucose value in weeks 2nd,6,10.Orbital blood detected TG,HDL,CREA and UREA by anesthetized rats in week10 th,We take the weight of the left kidney of rats to calculate kidney hypertrophy Index in week 10 th.To observe the morphological changes of rat kidney by HE staining,.(2)Renal expression of CTGF were measured by immunohistochemistry method,at the same time,IMS image analysie system is used to analyze the positive rate and average light density of CTGF.Results: 1.Compared to the normal group,blood Glucose in rats of model Group and SP group was significantly higher(P<0.05),diabetic model was successful,although the blood glucose values tends to decrease in SP group,but there was no significant difference compared with model group(P>0.05).The sp group and the model group `s mALB was significantly higher the normal group(P<0.01)in weeks 2nd,6,10,SP group were significantly lower than model group(P<0.01).There was significant difference of UREA,CREA among the three groups(P<0.05),Compared with the normal group,CREA of SP group was Marked diminish(P<0.01),UREA of SP group was lower(P<0.05).Compared to the normal group,TG in rats of model Group and SP group was significantly higher(P<0.01),HDL in rats of model Group and SP group was significantly lower(P<0.05).TG of SP group was lower than normal group,HDL of SP group was higher than normal group.Compared to the normal control group,body weight of rats in model Group and SP group decreased significantly(P<0.05),the rats body weight of the SP group were higher than the model group(P<0.05).Kidney hypertrophy Index of the model group and the SP group were significant highter than the normal group(P<0.05),and there was significant difference between SP group and model group(P<0.05).The morphologic of rat kidney tissue slices had been changed in SP group and normal group,glomerular was normal,Glomerular epithelial cell vacuolation minor.2.CTGF protein expression in model group were significantly higher than in normal group,concentrated in the proximal convoluted tubules and distal convoluted tubules,expression of CTGF was inhibited in SP group,glomeruli are basically normal,CTGF protein positive area and the mean optical density in the SP group were significantly lower than in model group(P<0.05),there is no significant difference compared with the normal group.Conclusions:(1)Shiweijiangtang pulvis reduce mALB,CREA,UREA,TG and kidney hypertrophy Index.HDL cholesterol and the weight of DN rats were increased,inhibition of glomerular and renal tubular lesions,protective effects on the kidneys.(2)Shiweijiangtang pulvis can inhibit CTGF expression of kidney in DN rats and slow renal fibrosis. |
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