A Exploration Of The T Cell Receptor Immune Repertoire Molded By HIV Vaccines

The traditional method to evaluate the vaccine is the immune success rate and population immunity levels monitoring.The last traditional way is to do test at the protein level.The sample size of traditional method is can't be very big,and the universality and diversity of data can't be verified.What's more,the genetic material basis and the immune response between individual is different.As a result,the traditional method cannot distinguish between different individual immune response,more can't explain the phenomenon from the essence.By which to analyze the effect of injecting the vaccine,can the stand or fall of vaccine in nature genetics,vaccine evaluation method has more advantages than others.The paper injected with the vaccine of HIV-1 will be Indian rhesus monkey samples which were built libraries of the T cell receptor beta chain(TRB)immune repertoires,and analysis and evaluation of the vaccine.The results showed that all three vaccines elicited an immune response on the 7th day after injection and had clonal expansion.This shows that at the DNA level,it is possible to reflect whether the vaccine injection can cause the immune system reaction,and the change of the number of the amplified clones also reflects the change of the immune response.The analysis of the change of the immune response is mainly made before and after the injection of the vaccine.At the level of DNA,the similarity and diversity of samples were compared based on the nature of the antigen-antibody binding diversity,and the patterns of changes were analyzed.The changes of different clonal abundance were used as evaluation indexes of vaccine efficacy.Can essentially distinguish between individual differences,but also to guide the vaccine development and transformation.